Plasmid for detecting mercury in water

Details Plasmid for detecting mercury in water Plasmid for detecting mercury in water

1993-08-23

1996-11-05

Hendricks, Keith D.

Chemistry: molecular biology and microbiology

Vector, per se

435189, 536 232, 536 241, C12N 1563, C12N 902, C12N 1553

Patent

active

055717225

ABSTRACT:
Devices for the detection of small quantities either of divalent inorganic mercury ion (Hg.sup.2+) or of both Hg.sup.2+ and monomethyl mercury in water are disclosed. These devices comprises a bioluminescence detecting means and a bioluminescent biosensory microorganism cells, that emit significant light only when exposed to either to Hg.sup.2+ or to Hg.sup.2+ or momomethyl mercury. Plasmid cassettes and host microorganisms containing such cassettes are also disclosed The plasmid cassettes comprises a lux gene operon complex from Xenorhabdus luminescens. The lux operon complex comprises luxC, luxD, luxA, luxB, and luxE genes but is free of (1) a promoter for the complex and (2) an inducible regulatory gene for the complex.

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