Plasmid for delivery of nucleic acids to cells and methods of us

Details Plasmid for delivery of nucleic acids to cells and methods of us Plasmid for delivery of nucleic acids to cells and methods of us

1995-06-07

1998-06-09

Jones, W. Gary

Chemistry: molecular biology and microbiology

Vector, per se

514 44, 435 6, 935 52, 935 55, 935 56, 935 22, 935 23, C12N 1563, A61K 3500, C12Q 168

Patent

active

057632707

ABSTRACT:
This invention relates to plasmid constructs useful for nonviral human gene therapy. The plasmid constructs incorporate plasmid elements for achieving high copy number, avoiding plasmid instability an providing a plasmid selection process. These constructs can be used to deliver nucleic acids to cells in vehicles which are compatible with human therapeutic use.

REFERENCES:
patent: 4956288 (1990-09-01), Barsoum
patent: 5019506 (1991-05-01), Daly et al.
patent: 5160489 (1992-11-01), Lukacsovich et al.
patent: 5308760 (1994-05-01), Brown et al.
Hodgson, The Vector Void in Gene Therapy, Biotechnology vol. 13 pp. 222-225, 13 Mar. 1995.
Culver et. al., Gene Therapy for Cancer, Tig, vol. 10, No. 5, pp. 174-178, May 1994.
Marshall, Gene Therapy's Growing Pains, Science, vol. 269., pp. 1050-1055, 25 Aug. 1995.
Crouzet et al., "Construction of a broad-host-range non-mobilized stable vector carryin RP4 par-region," Gene 110;105-108 (1992).
Aslandis and Schmitt, "Regulatory Elements of the Raffinose Operon: Nucleotide Sequences of Operator and Repressor Genes," J. Bacteriol. 172:2178-2180 (1990).
Barker et al., "Sequence and Properties of operator mutations in the bio operon of Escherichia coli,"Gene 13:89-102 (1981).
Bautista and Graham, "Insertional mutagenesis using a synthetic lac operator," Gene 82:201-208 (1989).
Benner-Luger and Boos, "The mglB sequence of Salmonella typhimurium LT2; promoter analysis by gene fusions and evidence for a divergently oriented gene coding for the mgl repressor," Mol. Gen. Genet. 214:579-587(1988).
Bentley et al., "Plasmid-Encoded Protein: The Principal Factor in the Metabolic Burden Associated with Recombinant Bacteria," Biotechnology and Bioengineering 35:668-681 (1990).
Brosius, J., "Toxicity of an overproduced foreign gene product in Escherichia coli and its use in plasmid vectors for the selection of transcription terminators," Gene 27:161-172 (1984).
Brosius and Holy, "Regulation of ribosomal RNA promoters with a synthetic lac operator," Proc. Natl. Acad. Sci. USA 81:6929-6933 (1984).
Chen et al., "Construction and characterization of a novel cross-regulation system for regulating cloned gene expression in Escherichia coli,"Gene 130:15-22 (1993).
Cornet et al., "Plasmid psC101 Harbors a Recombination Site, psi, Which is Able to Resolve Plasmid Multimers and To Substitute for the Analogous Chromosomal Escherichia coli Site dif," J. Bacteriology 176:3188-3195 (1994).
Danner, D., "The lac operator as a phenotypc label for DNA fragments cloned in Escherichia coli," Gene 44:193-199 (1986).
Diederich et al., "New Cloning Vectors for Integration into the .lambda. Attachment Site attB c the Escherichia coli Chromosome," Plasmid 28:14-24 (1992).
Eberl et al., "Analysis of the multimer resolution system encoded by the parCBA operon of broad-host-range plasmid RP4," Molecular Microbiology 12:131-141 (1994).
Engels and Meyers, "Inactivation of the Transcriptional-Dependent Inhibition of Plasmid Replication: A Selection Method for Cloning Large DNA Fragments," BioTechniques 14:324-325 (1993).
Fitzwater et al., "Conditional high copy number ColE1 mutants: resistance to RNA1 inhibition in vivo and in vitro," EMBO J 7:3289-3297 (1988).
Fritz et al., "Characterization of two mutations in the Escherichia coli gene inactivating the second galactose operator and comparatively studies of repressor binding," EMBO J. 2:2129-2135 (1983).
Fujita and Fujita, "Identification and nucleotide sequence of the promoter region of the Bacillus subtilis gluconate operon," Nucleic Acid Research 14:1237-1252 (1986).
Ghersa et al., "Commonly used cat reporter vectors contain a cAMP-inducible, cryptic enhancer that co-operates with NF-.sub.K B-sites," Gene 151:331-332 (1994).
Giza and Huang, "A self-inducing runaway-replication plasmid expression system utilizing the Rop protein," Gene 78:73-84 (1989).
Hammer et al., "DNA specifity of Escherichia coli deoP1 operator-DeoR repressor recognition," Mol. Gen. Genet. 237:129-133 (1993).
Hiraga, "Chromosome and Plasmid Partition in Escherichia Coli," Ann. Rev. Biochem. 61:283-306 (1992).
Huong et al., "Molecular Cloning and Physical and Functional Characterization of the Samonella typhimurium and Salmonella typi Galactose Utilization Operons," J. Bacteriology 172:4392-4398 (1990).
Jensen et al., "Partitioning of Plasmid R1: The parA Operon is Autoregulated by ParR and Its Transcription is Highly Stimulated by a Downstream Activating Element," J. Mol. Biol. 236:1299-1309 (1994).
Kilstrup et al., "Nucleotide sequence of the carA gene and regulation of the carAB operon in Salmonella typhimurium," Eur. J. Biochem. 176:421-429 (1988).
Kushner et al., "Eukaryotic Regulatory Elements Lurking in Plasmid DNA: The Activator Protein-1 Site in pUC," Molecular Endocrinology, 8:405-407 (1994).
Latour and Weiner, "Regulation of in vitro expression of Escherichia coli frd operon: alanine and Fnr represent positive and negative control elements," Nucleic Acids Research 16:6339-6352 (1988).
Leite et al., "Negative effect of a cis-acting pBR322 element on adenovirus E1a gene expression," Gene 82:351-356 (1989).
Linder, et al., "Regulation of xylanolytic enzymes in Bacillus subtilis," Microbiology 140:753-757 (1994).
Lusky and Botchan, "Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences," Nature 239:79-81 (1981).
Lutz et al., "Syrinx 2A: An improved .lambda. phage vector designed for screening DNA libraries by recombination in vivo," Proc. Natl. Acad. Sci. USA 84:4379-4383 (1987).
Mata-Gilsinger et al., "Characterization of the Operator Siters of the EXU Regulon in Escherichia coli K-12 by Operator-Constitutive Mutations and Repressor Titration," Genetics 105:829-842 (1983).
Naik and Hassan, "Use of site-directed mutagenesis to identity an upstream regulatory sequence of sodA gene of Escherichia coliK-12," Proc. Natl. Acad. Sci. USA 87:2618-2622 (1990) (39).
Oskouian and Stewart, "Cloning and Characterization of the Repressor Gene of the Staphylococcus aureus Lactose Operon," J. Bacteriology 169:5459-5465 (1987).
Osuna et al., "Identification of the hutUH Operator (hutUo) from Klebsiella aerogenes by DNA Deletion Analysis," J. Bacteriology 176:5525-5529 (1994).
Park et al., "Two-Stage Fermentation with Bacteriophate .lambda. as an Expression Vector in Escherichia coli," Biotechnology and Bioengineering 37:297-302 (1991).
Peretti and Bailey, "Simulations of Host-Plasmid Interactions in Escherichia coli: Copy Number, Promoter Strength, and Ribosome Binding Site Strength Effects on Metabolic Activity and Plasmid Gene Expression," Biotechnology and Bioengineering 29:316-328 (1987).
Peterson et al., "Context-Dependent Gene Expression: cis-Acting Negative Effects of Specific Procaryotic Plasmid Sequences on Eucaryotic Genes," Mol. Cell. Biol. 7:1563-1567 (1987).
Porter et al., "Use of the Escherichia Coli SSB Gene to Prevent Bioreactor Takeover by Plasmidless Cells," Biotechnology 8:47-51 (1990).
Salmaron et al., "Interaction between Transcriptional Activator Protein LAC9 and Negative Regulatory Protein GAL80," Mol. Cell. Biol. 9:2950-2956 (1989).
Simons et al., Possible ideal lac operator: Escherichia coli lac operator-like sequences from eukaryotic genomes lack the central G-C pair, Proc. Natl. Acad. Sci. USA 81:1624-1628 (1984).
Smith, "Regulatory considerations for nucleic acid vaccines," Vaccine 12(16):1515-1519 (1994).
Sogaard-Andersen et al., "Tandem DNA-bound cAMP-CRP complexes are required for transcriptional repression of the deoP2 promoter by the CytR repressor in Escherichia coli,"Mol. Microbiol. 4;1595-1601 (1990).
Stragier et al., "Regulatory Pattern of the Escherichia coli lysA Gene: Expression of Chromosomal lysA-lacZ Fusions," J. Bacteriology 156:1198-1203 (1983).
Summers et al., "Multimer resolution systems of ColE1 and ColK: localisation of the crossover site," Mol. Gen. Genet. 201:334-338 (1985).
Tully and Cidlowski, "pBR322 Contains Glucocorticoid Regulatory Element DNA Consensus Sequences," Biochem. Biophys. Res. Commun. 144:1-10 (1987).
Uhlin et al., "A Runaway-Replication Mutant of Plasmid R1drd-19: Temperature-Dep

Affiliated with

Also associated with

Rating

Plasmid for delivery of nucleic acids to cells and methods of us does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Plasmid for delivery of nucleic acids to cells and methods of us, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Plasmid for delivery of nucleic acids to cells and methods of us will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2198227