Plasmid containing a gene for tetracycline resistance and DNA fr

Chemistry: molecular biology and microbiology – Vector – per se

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4351723, 43525232, 435843, 536 27, 935 29, C12N 1577, C12N 1565, C12N 1500

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active

051588919

ABSTRACT:
A plasmid and a DNA fragment each of which contains a gene for tetracycline resistance derived from a glutamic acid-producing coryneform bacterium, and a coryneform bacterium containing said DNA fragment. The gene for tetracycline resistance derived from a glutamic acid-producing coryneform bacterium is a useful selective marker in effecting the breeding of a glutamic acid-producing coryneform bacterium by genetic recombination. Using the plasmid, the DNA fragment or the coryneform bacterium of the present invention, breeding of the glutamic acid-producing coryneform bacterium can be easily and effectively conducted by means of recombinant DNA technique.

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Foster, T. J., 1983, Microbiological Reviews, pp. 361-409.
Bolivar et al. (1977), Gene, vol. 2, pp. 95-113.
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Rockhill et al., Antimicrobial Agents and Chemotherapy, vol. 21, pp. 842-843, 1982 (May).
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