Plasmid cloning vector for Bacillus subtilis

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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435317, 435172, C12N 120, C12N 100, C12N 1500

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active

044194501

ABSTRACT:
A novel chemical compound, plasmid pOS4, is prepared by joining fragments of the plasmids pSE3 and pE194. The pOS4 plasmid, containing kanamycin resistance and erythromycin resistance coding genes, is useful as a cloning vehicle in recombinant DNA work.

REFERENCES:
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Gryczan, et al., J. Bacteriology, 141, 246-253, (1980).
Weisblum, et al., J. Bacteriology, 137, 635-643, (1979).
Polak, et al., Plasmid, 7, 152-162, (1982).
Kreft, et al., Molec. Gen. Genet., 162, 59-67, (1978).
Keggins, et al., Proc. Natl. Acad. Sci., U.S.A., 75, 1423-1427, (1978).
Gryczan, et al., Proc. Natl. Acad. Sci., U.S.A., 75, 1428-1432, (1978).

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