Plasmid cloning and expression vectors for use in microorganisms

Details Plasmid cloning and expression vectors for use in microorganisms Plasmid cloning and expression vectors for use in microorganisms

1982-12-13

1987-01-06

Martinell, James

Chemistry: molecular biology and microbiology

Miscellaneous

435 68, 435 90, 435 91, 4351723, 435253, 935 29, 935 39, 935 40, 935 41, 935 42, 935 43, 935 44, 935 45, 935 46, C12N 100, C12N 1500, C12N 120, C12P 2100, C12P 2102, C12P 1934

Patent

active

046346783

ABSTRACT:
DNA cloning and expression vectors capable of replication in a microbial host comprising from upstream to downstream (a) at least one promoter; (b) a translation start codon; (c) a cloning segment which provides a means for inserting nucleic acid sequences and (d) a sequence coding for a detectable gene product, out of translational phase with the translation start codon but capable of being readjusted to the translational phase of said start codon by insertion into the cloning segment of nucleic acid sequences containing the proper number of nucleotides for readjustment, said gene product providing a means for detecting expression of inserted nucleic acid sequences.

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