Plasmid bearing a cDNA copy of the genome of bovine viral diarrh

Details Plasmid bearing a cDNA copy of the genome of bovine viral diarrh Plasmid bearing a cDNA copy of the genome of bovine viral diarrh

1997-05-21

1999-12-14

Wortman, Donna

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 9121, 435 9133, 4352351, 4353201, C12N 1500, C12N 700

Patent

active

060016132

ABSTRACT:
A plasmid bearing a cDNA copy of the genome of bovine viral diarrhea virus (BVDV), chimeric derivatives of the plasmid and a method of producing an infectious bovine viral diarrhea virus using the plasmid are disclosed. The invention relates to a plasmid DNA molecule that replicates easily in E. coli and contains a sufficient portion of the genome of BVDV, cloned as cDNA, to be a suitable template to produce RNA in vitro which, upon transfection into bovine cells, gives rise to infectious BVDV. The BVDV created by the process of the invention can be engineered for use as a vector in many advantageous applications.

REFERENCES:
patent: 4719177 (1988-01-01), Baltimore et al.
patent: 4992367 (1991-02-01), Cullen
patent: 5206163 (1993-04-01), Renard et al.
patent: 5670152 (1997-09-01), Weiner et al.
Brock et al., Journal of Virological Methods, 38:39-46, 1992.
Collett, et al., Virology, 165:191-199, 1988.
Ferrari, M., Microbiologica, 8:17-22, 1985.
Elaine T. Schenborn, et al., "A Novel Transcription Property of SP6 and T7 RNA Polymerases: Dependence on Template Structure," Nucleic Acids Res., vol. 13, No. 17, 1985, pp. 6223-36.
Ruitang Deng, et al., "5' and 3' Untranslated Regions of Pestivirus Genome: Primary and Secondary Structure Analyses," Nucleic Acids Res., vol. 21, No. 8, 1993, pp. 1949-1957.

Affiliated with

Also associated with

Rating

Plasmid bearing a cDNA copy of the genome of bovine viral diarrh does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Plasmid bearing a cDNA copy of the genome of bovine viral diarrh, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Plasmid bearing a cDNA copy of the genome of bovine viral diarrh will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-862206