Plasmid and uses thereof

Details Plasmid and uses thereof Plasmid and uses thereof

1988-09-08

1991-05-28

Teskin, Robin L.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4352523, 4352524, 4353201, C12N 1500, C12N 120

Patent

active

050195069

ABSTRACT:
Plasmids carrying one or more genes which confer phage insensitivity upon Streptococcus lactis or Streptococcus lactis subsp. diacetylactis as well as Streptococcus cremoris bacteria in which the insensitivity is not destroyed at temperatures of up to 40.degree. C. are described. DNA fragments are also described which encode phage insensitivity. Such plasmids and DNA fragments are suitable for conferring phage insensitivity upon bacteria or food starter cultures containing bacteria.

REFERENCES:
Klaenhammer, T. R. and R. B. Sanozky, Conjugal Transfer from Streptococcus Lactis ME2 of Plasmids Encoding Phage Resistance, Nisin Resistance and Lactose-Fermenting Ability: Evidence for a High-frequency Conjugative Plasmid Responsible for Abortive Infection of Virulent Bacteriophage, Journal of General Microbiology (1985), 131, pp. 1531-1541.
Steenson, L. R. and T. B. Klaenhammer, Streptococcus Cremoris M12R Transconjugants Carrying the Conjugal Plasmid pTR2030 are Insensitive to Attack by Lytic Bacteriophages, Applied and Environmental Microbiology, Oct. 1985, vol. 50, No. 4, pp. 851-858.
Sing, W. D. and T. R. Klaenhammer, Conjugal Transfer of Bacteriophage Resistance Determinants on pTR2030 into Streptococcus cremoris Strains, Applied and Environmental Microbiology, Jun. 1986, vol. 51, No. 6, pp. 1264-1271.
de Vos et al., FEMS Microbiol. Lett. 23:175-178, 1984. Microbiology,
Jarvis, A. W. and T. R. Klaenhammer, Bacteriophage Resistance Conferred on Lactic Streptococci by the Conjugative Plasmid pTR2030: Effects on Small Isometric-, Large Isometric-, and Prolate-Headed Phages . Applied and Environmental Microbiology, Jun. 1986, vol. 51, No. 6, pp. 1272-1277.
Jarvis, A. W. and T. R. Klaenhammer, Bacteriophage Resistance Plasmid pTR2030 Inhibits Lytic Infection r.sub.1 t Temperate Bacteriophage but Not Induction of r.sub.1 t Prophage in Streptococcus Cremoris R1. Applied and Environmental Microbiology, Feb. 1987, vol. 53, No. 2, pp. 385-389.
Sanders, M. E., P. J. Leonhard, W. D. Sing and T. R. Klaenhammer, Conjugal Strategy for Construction of Fast Acid-Producing, Bacteriophage-Resistant Lactic Streptococci for Use in Dairy Fermentations. Applied and Environmetal Microbiology, Nov. 1986, vol. 52, No. 5, pp. 1001-1007.

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