Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1979-03-05
1981-06-16
Tanenholtz, Alvin E.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
435 91, 435317, 435820, 435886, C12N 120
Patent
active
042738750
ABSTRACT:
A novel chemical compound, essentially pure plasmid pUC6, which is obtainable from a biologically pure culture of the microorganism Streptomyces espinosus biotype 23724a, NRRL 11439. The pUC6 plasmid is useful as a cloning vehicle in recombinant DNA work. For example, using DNA methodology, a desired gene, for example, the insulin gene, can be inserted into pUC6 and the resulting plasmid can then be transformed into a suitable host microbe which, upon culturing, produces the desired insulin.
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J. Antibiotics 30, 1146-1149, 897-899 (1977).
Nature 254, 265-267 (1975).
J. Gen. Microbiol 98, 239-252 (1977).
J. Antibiotics 33, 45-47 (1969).
Broda, Plasmids, Freeman and Company Ltd., p. 5-11 (1979).
Science, vol. 196, Editorial page, Apr. 1977.
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Huber et al., Can. J. Microbiol 24, 631-632 (1978).
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Biomedicine 26, 236-249 (1977).
J. Gen. Microbiol 90, 336-346 (1975).
Saliwanchik Roman
Tanenholtz Alvin E.
The Upjohn Company
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