Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1992-07-14
1993-06-15
Russel, Jeffrey E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
530384, 530413, 530416, 530417, 530420, C07K 1506, C07K 320, C07K 324
Patent
active
052199959
ABSTRACT:
The present invention describes a process for activating Factor II to Factor II.sub.a by incubating Factor II in the presence of Factor V, Factor X.sub.a, phospholipids, and calcium ions. Each of the factors is prepared from a single impure protein fraction which includes Factors II, V and X. The Factor II, V and X purification procedure comprises the steps of DEAE ligand chromatography and precipitation by the addition of barium chloride. Factor V is recovered from the barium chloride supernatant, and Factors II and X are contained in the barium chloride precipitate. The barium chloride precipitate is dissolved in an aqueous solution and is applied to a chromatographic resin coupled with a ligand which binds Factor X and Factor II weakly or not at all. Factor II is recovered from the fraction, which remains unbound or weakly bound to the Factor X binding ligand. Factor X.sub.a is prepared by recovering Factor X from the ligand during the Factor II preparation procedure and activating the Factor X to Factor X.sub.a by specific proteolytic cleavage.
REFERENCES:
patent: 4725673 (1988-02-01), Herring
Fasco et al., "Human .alpha.-, .beta.(.sub.T)-, .gamma.(.sub.T)-Thrombins," Div. Lab. and Res., New York State Dept. of Health, Albany, N.Y. 12201 (Abstract) Physiology, 765.
Mannis et al., "Uses of Thrombin in Ocular Surgery: Effect on the Corneal Endothelium," Arch. Ophthalmol., 106, 251-253 (1988).
Krishnaswamy et al., "Activation of Human Prothrombin by Human Prothrombinase: Influence of Factor Va on the Reaction Mechanism," J. Biol. Chem., 262, 3291-3299 (1987).
Helmkamp et al., "Effectiveness of topical hemostatic agents," Contemporary OB/GYN, 171-180 (1985).
Ghosh et al., "Note on the Preparation of Bovine Thrombin," Thrombosis Research, 20, 281-283 (1980).
Pepper et al., "Chromatography of Human Prothrombin Complex on Dextran Sulphate Agarose," Thrombosis Research, II, 687-692 (1977).
Fenton et al., "Human Thrombins: Production, Evaluation, and Properties of .alpha.-Thrombin," J. Biol. Chem., 252, 3587-3598 (1977).
Lundblad, Roger L., "A Rapid Method for the Purification of Bovine Thrombin and the Inhibition of the Purified Enzyme with Phenylmethylsulfonyl Fluoride," Biochem., 10, 2501-2506 (1972).
Denson et al., "The Specific Assay of Prothrombin Using the Taipan Snake Venom," Brit. J. Haematol., 21, 219-226 (1971).
Seegers et al., "Preparation and Properties of Thrombin," Arch. Biochem. & Biophys., 128, 194-201 (1968).
Seegers et al., "Purification of Prothrombin and Thrombin by Chromatography on Cellulose," Can. J. Biochem. Physiol., 38, 1405-1410 (1960).
Tidrick et al., "Clinical Experience with Thrombin as an Hemostatic Agent," Surgery, 14, 191-196 (1943).
Herring Steven W.
Noda Munehiro
Shitanishi Kenneth T.
Uemura Yahiro
Alpha Therapeutic Corporation
Russel Jeffrey E.
Touzeau P. Lynn
LandOfFree
Plasma fraction purification does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Plasma fraction purification, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Plasma fraction purification will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1044056