Plasma and hemoglobin-associated acetaldehyde as a marker of alc

Details Plasma and hemoglobin-associated acetaldehyde as a marker of alc Plasma and hemoglobin-associated acetaldehyde as a marker of alc

1987-08-07

1989-03-21

Richman, Barry S.

Chemistry: analytical and immunological testing

Oxygen containing

Carbonyl, ether, aldehyde or ketone containing

436132, 436161, 436172, G01N 3348

Patent

active

048142808

ABSTRACT:
An improved fluorigenic, high-performance, liquid chromatography (HPLC) assay comprises reacting plasma and hemolysate samples with cyclohexanedione in the presence of ammonium ion to form a methylacridine dione fluorigenic species that is stable and exhibits high recovery values. Acetaldehyde content was determined by comparing the fluorescent peaks of the HPLC eluted band with standard peaks. Alcohol intake by the subject was detected by comparing the acetaldehyde levels to those of teetotalers.

REFERENCES:
Stevens et al., Acetaldehyde Adducts with Hemoglobin, J. Clin. Invest. vol. 67 Feb. 81, pp. 361-369.
Homaidan et al., Acetaldehyde Hemoglobin Adducts: An Unreliable Marker of Alcohol Abuse, Clin. Chem. 30/3, 480-482 (1984).
Stahovec et al., Trace Analysis of Aldehydes by Precolumn Fluorigenic Labeling with 1,3-Cyclohexanedione and Reversed-Phase H.P.L.C., J. of Chromatography 298 (1984) 399-406.

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