Plants with reduced activity of a class 3 branching enzyme

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide encodes an inhibitory rna molecule

Reexamination Certificate

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Details

C800S286000, C800S295000, C800S298000, C435S320100, C435S468000, C435S200000, C435S201000, C536S023100, C536S023200, C536S023600, C536S024500

Reexamination Certificate

active

07626080

ABSTRACT:
The present invention relates to plant cells and plants, which are genetically modified, wherein the genetic modification leads to the reduction of the activity of a Class 3 vegetable branching enzyme in comparison with corresponding wild type plant cells or wild type plants that have not been genetically modified. Furthermore, the present invention relates to means and methods for the manufacture of such plant cells and plants. Plant cells and plants of this type synthesise a modified starch. The present invention therefore also relates to the starch synthesised by the plant cells and plants according to the invention as well as to methods for the manufacture of the starch and to the manufacture of starch derivatives of this starch. Furthermore, the present invention relates to nucleic acids coding a Class 3 branching enzyme, vectors, host cells, plant cells and plants containing such nucleic acid molecules.

REFERENCES:
patent: WO 96/34968 (1996-11-01), None
patent: WO9634968 (1996-11-01), None
patent: WO 01/70942 (2001-09-01), None
patent: WO0170942 (2001-09-01), None
Tetlow et al 2004 Journal of Experimental Botany 55(406):2131-2145.
Der Hoeven et al 2001 GenBank Accession BG886850.
Blauth et al. (Mar. 2001) “Identification ofMutatorInsertional Mutants of Starch-Branching Enzyme 2a in Corn.”Plant Physiology125: 1396-1405.
Blauth et al. (2002) “Identification ofMutatorinsertional mutants of starch-branching enzyme 1 (sbe1) inZea maysL.”Plant Molecular Biology48: 287-297.
Flipse et al. (1996) “Introduction of sense and antisense cDNA for branching enzyme in the amylose-free potato mutant leads to physico-chemical changes in the starch.”Planta198: 340-347.
Jobling et al. (1999) “A minor form of starch branching enzyme in potato (Solanum turberosumL.) tubers has a major effect on starch structure: cloning and characterisation of multiple forms ofSBE A.” The Plant Journal18(2): 163-171.
Khoshnoodi et al. (1996) “The multiple forms of starch-branching enzyme I inSolanum tuberosum.” Eur. J. Biochem242: 148-155.
Larsson et al. (1998) “Molecular Cloning and characterization of starch-branching enzyme II from potato.”Plant Molecular Biology37: 505-511.
Mizuno et al. (Sep. 5, 1993) “Alteration of the Structural Properties of Starch Components by the Lack of an Isoform of Starch Branching Enzyme in Rice Seeds.”The Journal of Biological Chemistry268(25): 19084-19091.
Rydberg et al. (2001) “Comparison of starch branching enzyme I and II from potato.”Eur. J. Biochem. 268: 6140-6145.
GenBank Accession No. BG886850 (Jun. 8, 2001).
GenBank Accession No. Q8GWK4 (Mar. 1, 2003).
GenBank Accession No. AK118785 (Dec. 13, 2002).
EPO Communication issued Feb. 16, 2009 in EPO Application No. 04 787 074.6.
UniProtKB/Swiss-Prot Q8U8L4 (GLGB—AGRT5) downloaded from http://www.uniprot.org/uniprot/Q8U8L4, 8 pgs, dated Jun. 23, 2009.
UniProtKB/TrEMBL Q82AS4 (Q82AS4—STRAW) downloaded from http://www.uniprot.org/uniprot/Q82AS, 9 pgs, dated Jun. 23, 2009.

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