Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters carbohydrate production in the plant
Reexamination Certificate
2005-03-15
2005-03-15
Fox, David T. (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters carbohydrate production in the plant
C800S286000, C800S298000, C800S306000, C800S312000, C800S317200, C800S317300, C800S320000, C800S320100, C800S320200, C800S320300, C435S419000, C435S421000
Reexamination Certificate
active
06867350
ABSTRACT:
The subject invention concerns materials and methods for enhancing starch production in plants. Starch production is enhanced, relative to levels observed in wildtype or control plants, by reduction of the plant 14-3-3 protein(s) which subsequently results in increased accumulation of starch in the plant. In one embodiment, the 14-3-3 protein expression is reduced using polynucleotides that are antisense to the 14-3-3 gene sequences expressed in the plant. In another embodiment, the 14-3-3 protein expression is reduced by “knockout” of a 14-3-3 gene or gene sequences. The subject invention also pertains to transformed and transgenic plants that have polynucleotides that are antisense to the 14-3-3 gene sequences expressed in the plant, wherein the transformed and transgenic plants exhibit enhanced starch production. The subject invention also pertains to “knockout” plants in which the normal functional 14-3-3 gene in the plant is deleted or replaced with a non-functional form of the gene. The subject invention also concerns the “antisense” polynucleotides of the invention that when introduced into a plant cell can function to effectively reduce expression of the 14-3-3 proteins in a plant.
REFERENCES:
Roberts et al., Fusicoccin, 14-3-3 Proteins and Defense Responses in Tomato Plants. Plant Physiology (1999); vol. 119, pp. 1243-1250.*
Wu K. et al., Plant Physiology, 1997, vol. 114, pp. 1342-1431.*
Elomaa P. et al., Molecular Breeding 1996, vol. 2, pp. 41-50.*
Waterhouse P. et al., Trends in Plant Sciences, Nov. 1999, vol. 4, No. 11 pp. 452-457.*
Smith C. et al., Nature 1988, 334: 724-726.*
Wilczynski G. et al. J. of Plant Physiol, 1998, vol. 153, pp. 118-126.*
Gordon-Kamm et al. The Plant Cell, Jul. 1990, vol. 2, pp. 603-618.*
Ferl et al. GenBank Accession No. U36446, submitted Sep. 15, 1995.*
Ferl et al. GenBank Accession No. U60444, submitted Jun. 11, 1996.*
Wilczynski G. et al., J. of Pl. Phsiol., 1997, vol. 151, pp. 689-698.*
Altschul, S. F., et al., “Gapped BLAST and PSI-BLAST: A New Generation of Protein Database Search Programs”Nucleic Acids Research,1997, pp. 3389-3402, vol. 25, No. 17, Oxford University Press.
Bachmann, M. et al. “The Inhibitor Protein of Phosphorylated Nitrate Reductase from Spinach (Spinacia Oleracea) Leaves in a 14-3-3 Protein”FEBS Letters,1996, pp. 127-131, vol. 387.
Bechtold, N. et al. “In Planta Agrobacterium-Mediated Transformation of AdultArabidopsis ThalianaPlants by Vacuum Infiltration”Methods in Molecular Biology,1998, pp. 259-266, vol. 82, Humana Press Inc., Totowa, NJ.
Bihn, E. A. et al. “Localization of 14-3-3 Poteins in the Nuclei of Arabidopsis and Maize”The Plant Journal,1997, pp. 1439-1445, vol. 12, No. 6.
Cao, H. et al., “Identification of the Soluble Starch Synthase Activities of Maize Endosperm”Plant Physiology,1999, pp. 205-215, vol. 120.
Chung, H. J. et al. “The 14-3-3 Proteins: Cellular Regulators of Plant Metabolism”Trends in Plant Science,1999, pp. 367-371, vol. 4.
Craig, J. et al. “Mutations in the Gene Encoding Starch Synthase II Profoundly Alter Amylopectin Structure in Pea Embryos”The Plant Cell,1998, pp. 413-426, vol. 10.
Daugherty, C. J. et al. “Molecular Organization and Tissue-Specific Expression of an Arabidopsis 14-3-3 Gene”The Plant Cell,1996, pp. 1239-1248, vol. 8.
De Vetten N. C. et al. “Two Genes Encoding GF14 (14-3-3) Proteins inZea mays”Plant Physiology,1994, pp. 1593-1604, vol. 106.
Edwards, A. et al. “A Combined Reduction in Activity of Starch Synthases II and III of Potato has Novel Effects on the Starch of Tubers”The Plant Journal,1999, pp. 251-261, vol. 17, No. 3.
Holsters, M. et al. “Transfection and Transformation ofAgrobacterium Tumefaciens”Molec. Gen. Genet.,1978, pp. 181-187, vol. 163.
Imparl-Radosevich, J. M., et al. “Analysis of Purified Maize Starch Synthases lla and llb: SS Isoforms Can Be Distinguished Based on Their Kinetic Properties”Archives of Biochemistry and Biophysics,1999, pp. 131-138, vol. 362, No. 1.
Konishi, Y. et al. “Characterization of Starch Granules from Waxy, Nonwaxy, and Hybrid Seeds ofAmaranthus hypochondriacusL.”Agric. Biol. Chem.,1985, pp. 1965-1971, vol. 49, No. 7.
Krysan, P. J. et al. “Identification of Transferred DNA Insertions WithinArabidopsisGenes Involved in Signal Transduction and Ion Transport”Proc. Natl. Acad. Sci. USA,1996, pp. 8145-8150, vol. 93.
Lloyd, J. R. et al. “Simultaneous Antisense Inhibition of Two Starch-Synthase Isoforms in Potato Tubers Leads to Accumulation of Grossly Modified Amylopectin”Biochem. J.,1999, pp. 515-521, vol. 338.
MacDonald, F. D. et al. “Solubilization of the Starch-Granule-Bound Starch Synthase of Normal Maize Kernels”Plant Physiology,1983, pp. 175-178, vol. 73.
Moorhead, G. et al. “Phosphorylated Nitrate Reductase From Spinach Leaves is Inhibited by 14-3-3 Proteins and Activated by Fusicoccin”Current Biology,1996, pp. 1104-1113, vol. 6, No. 9.
Moorhead, G. et al. “Phosphorylation-dependent Interactions Between Enzymes of Plant Metabolism and 14-3-3 Proteins”The Plant Journal,1999, pp. 1-12, vol. 18, No. 1.
Mu-Forster, C. et al. “Physical Association of Starch Biosynthetic Enzymes with Starch Granules of Maize Endosperm”Plant Physiology,1996, pp. 821-829, vol. 111.
Mu-Forster, C. et al. “Surface Localization of Zein Storage Proteins in Starch Granules from Maize Endosperm”Plant Physiology,1998, pp. 1563-1571, vol. 116.
Preiss, J. et al. “Biochemistry, Molecular Biology and Regulation of Starch Synthesis”Genetic Engineering,1998, pp. 177-223, vol. 20.
Sehnke, P. C. et al. “Interaction of a Plant 14-3-3 Protein with the Signal Peptide of a Thylakoid-Targeted Chloroplast Precursor Protein and the Presence of 14-3-3 Isoforms in the Chloroplast Stroma”Plant Physiology,2000, pp. 235-241, vol. 122.
Sehnke, P. C. et al. “Plant Metabolism: Enzyme Regulation by 14-3-3 Proteins”Current Biology,1996, pp. 1403-1405, vol. 6, No. 11.
Sehnke, P. C. et al. “Regulation of Starch Accumulation by Granule-Associated Plant 14-3-3 Proteins”Proc. Natl. Acad. Sci. USA,2001, pp. 765-770, vol. 98. Also published online (www.pnas.org/cgi/doi/10.1073/pnas.021304198) before print on Jan. 9, 2001.
Smith, A. M. “Making Starch”Current Opinion in Plant Biology,1999, pp. 223-229, vol. 2.
Sokolov, L. N. et al. “Sugars and Light/Dark Exposure Trigger Differential Regulation of ADP-Glucose Pyrophosphorylase Genes inArabidopsis Thaliana(Thale Cress)”Biochem. J.,1998, pp. 681-687, vol. 336.
Sun, J. et al. “Modification of Carbon Partitioning, Photosynthetic Capacity, and O2Sensitivity in Arabidopsis Plants with Low ADP-Glucose Pyrophosphorylase Activity”Plant Physiology,1999, pp. 267-276, vol. 119.
Toroser, D. et al. “Site-Specific Regulatory Interaction Between Spinach Leaf Sucrose-Phosphate Synthase and 14-3-3 Proteins”FEBS Letters,1998, pp. 110-114, vol. 435.
Wu, K. et al. “The Arabidopsis 14-3-3 Multigene Family”Plant Physiology,1997, pp. 1421-1431, vol. 114.
Zeeman, S. C. et al. “A Starch-Accumulating Mutant ofArabidopsis ThalianaDeficient in a Chloroplastic Starch-Hydrolysing Enzyme”The Plant Journal,1998, pp. 357-365, vol. 15, No. 3.
Chung Hwa Jee
Ferl Robert J.
Hannah L. Curtis
Sehnke Paul C.
Wu Ke
Fox David T.
Kallis Russell
Saliwanchik Lloyd & Saliwanchik
University of Florida Research Foundation Inc.
LandOfFree
Plants with enhanced ability to produce starch and methods... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Plants with enhanced ability to produce starch and methods..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Plants with enhanced ability to produce starch and methods... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3443286