Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...
Patent
1999-03-03
2000-11-14
Fox, David T.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
800288, 435 691, 4353201, 435468, 435469, 536 2372, C12N 1534, C12N 1582, C12N 1583, C12N 1584
Patent
active
061472786
ABSTRACT:
The invention relates to novel plant plasmid vectors comprising geminivirus DNA or a portion thereof having inserted therein a heterologous DNA sequence or gene, to processes and DNA intermediates useful in producing said vectors and to methods utilizing such vectors to replicate and express heterologous DNA sequences or genes in plants. In some embodiments, methods and compositions are provided for Ti plasmid delivery of these novel vectors into plants. In other embodiments, methods and compositions are provided which allow for the generation of geminivirus DNA containing plant plasmids in stably transformed plants. In still other embodiments, methods and compositions are provided for replicating and expressing heterologous DNA sequences or genes in plants employing the geminivirus DNA containing vectors of the present invention without causing disease symptoms.
REFERENCES:
patent: 4407956 (1983-10-01), Howell
patent: 4536475 (1985-08-01), Anderson
Howarth et al. J. Gen. Virol. 70:2717-2727, 1989.
Hamilton et al. EMBO J. 3(9):2197-2205, 1984.
Hamilton et al. Nucleic Acids Res. 11(21):7387-7398, 1983.
Buck et al. Plant Mol. Biol. 2:351-354, 1983.
Hamilton et al. FEMS Microbiol. Lett. 11(4): 263-267, 1981.
Goodman, R. J. Gen. Viro. 54: 9-21, 1981.
Bisaro David
Brand Leslie
Elmer James Scott
Fraley Robert T.
Horsch Robert B.
Clayman Carol H.
Fox David T.
McBride Thomas P.
Monsanto Company
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