Plant VDE genes and methods related thereto

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...

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536 236, 536 231, 800298, 800288, 800290, 435 691, 435 701, 435471, 435410, 435418, 435419, 4353201, C12N 504, C12N 1529, C12N 1582, A01H 500

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060159391

ABSTRACT:
DNA sequences encoding plant vde enzymes are provided herein. The sequences may be joined to heterologous DNA sequences for use as probes and in DNA constructs to modify the genotype of a host organism. DNA constructs and methods are provided to modify a host cell phenotype by altering the amount of photoprotection enzyme present in the host cell. In plastid containing host cells, zeaxanthin levels and sensitivity to light can be modified through alterations in the level of vde enzymes.

REFERENCES:
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Tepperman and Dunsmuir. Plant Molecular Biology. 1990. Apr. issue. vol. 14: 501-511. Abstract enclosed.
Foyer et al. Plant Physiol. 1994. vol. 104: 171-178.
Hudspeth and Grula. Plant Molecular Biology. 1989. vol. 12:579-589.
Bugos, R.C., et al., "Molecular cloning of violaxanthin de-epoxidase from romaine lettuce and expression in Escherichia coli", Proceedings of the National Academy of Sciences vol. 93, No. 13 pp:6320-6325 (1996).
Rockholm, D.C., et al., "Violaxanthin de-epoxidase" Plant Physiology (1996) 110:697-703.
Yamamoto, H.Y., "Xanthophyll cycles" Methods in Enzymology, vol. 110, (1985) pp: 303-312.
Bugos, R.C., et al. "Arabidopsis thaliana violaxanthin de-epoxidase precursor" Abstract: XP002036888, (1996).
Bugos, R.C., et al. "Nicotiana tabacum violaxanthin de-epoxidase precursor" Abstract: XP002036887 (1996).

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