Plant transformation by gene transfer into pollen

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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4351721, 800205, 800DIG43, 800DIG56, C12N 1509

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active

056291833

ABSTRACT:
Plant germplasm is transformed with foreign DNA by introducing the DNA into pollen grains by a technique such as electroporation, mating ova of the desired plant line with the transformed pollen, and selecting for the transformed germplasm. The germinating pollen, resultant seed, and the progeny can each be screened for expression of the foreign gene. The transformed pollen can be used as a vector for introducing the foreign DNA into plant lines of similar or dissimilar origin, including both monocots and dicots.

REFERENCES:
patent: 4940835 (1990-07-01), Shan et al.
patent: 5049500 (1991-09-01), Arnizen et al.
patent: 5066594 (1991-11-01), DeBonte et al.
patent: 5384253 (1995-01-01), Kryzek et al.
Neumann, E., and Bierth, P., Gene Transfer by Electroporation, American Biotechnology Laboratory, publ. Monthly by International Scientific Communications, Fairfield, CN, vol. 4, No. 2, Mar./Apr. 1986, pp. 10-15.
Riggs, Charles Daniel, and Bates, George W., "Stable transformation of tobacco by electroporation: Evidence for plasmid concatenation", Proc. Natl. Acad. Sci. USA, vol. 83, Aug. 1986, Genetics, pp. 5602-5606.
Christou, Paul, et al., "Stable transformation of soybean by electroporation and root formation from transformed callus", Proc. Natl. Acad. Sci. USA, vol. 84, Jun. 1987, Applied Biology, pp. 3962-3966.
K. P. Mishra et al., "In vitro Electroporation of Tobacco Pollen," Plant Sci. 52: 135-139 (1987).
Stephen Berberich, "USDA Plant Molecular Biology Laboratory to Open," Research News (Mar. 25).
Mishra et al (1987) Plant Science 52: 135-139.
Ohta (1986) Proc. Natl. Acad. Sci., USA 83: 715-719.
Sanford (1988) in Forest and Crop Biotechnology: Progress and Prospects; Colloquium pp: 163-173 Springer Verlag, NY, 1988.
Negrutiu, et al "Pollen: International Conference.", Amherst, MA, USA, Jun. 1985, pp. 65-70.
Pan, et al (Mar. 31, 1989) Maize Genetics Cooperation Newsletter #63:20.
Potrykus (Jun. 1990) Bio/Technology 8:535-542.
Mathews et al (1990) Sex Plant Reprod. 3(3):147-151.
Abdul-Baki, et al (1990) Plant Science 70(2):181-190.
Negrutiu, I, et al., "Attempts to Transform for Kanamycin-Resistance in Mature Pollen of Tobacco," Biotechnology and Ecology of Pollen, Proceedings of the International Conference on the Biotechnology and Ecology of Pollen, 9-11 Jul. 1985, Univ. of Massachusetts, Amherst, MA, pp. 65-70.

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