Plant tissue comprising a subgenomic promoter

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...

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536 241, 536 231, 4351723, A01H 106, A01H 400, C12N 1582

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active

056334478

ABSTRACT:
A subgenomic promoter of a positive strand RNA virus is disclosed which directs the amplified expression of a structural gene in plant tissue. The core region and an upstream activating domain of the subgenomic promoter are identified. This promoter can be utilized in a modified virus, or in an appropriate engineered recombinant DNA derivative which may be chromosomally integrated or maintained as an episome in transformed cells.

REFERENCES:
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Ahlquist, P. and French, R. (1988) in RNA Genetics Book 2: RNA Variability, Chapter 3, Domingo et al. (eds.) CRC Press, Orlando, Florida.
French, R. et al. (1986) Science 231:1294-1297.
Ahlquist, P. et al. (1981) J. Mol. Biol. 153:23-38.
Miller, W. et al. (1985) Nature 313:68-70.
Marsh, L. et al. (1987) in Positive Strand RNA Viruses, A.R. Liss, Inc. (ed.) New York, pp. 327-336.
Ou, J.-H. et al. (1982) Proc. Natl. Acad. Sci. USA 79:5235-5239.
Barker, R. et al. (1983) Nucl. Acids Res. 11:2881-2891.
Janda, M. et al. (1987) Virology 158:259-262.
Ahlquist, p. and Janda, M. (1984) Mol. Cell. Biol. 4:2876-2882.

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