Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1992-11-10
1994-04-19
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435135, C12N 916, C12P 762
Patent
active
053044819
ABSTRACT:
This invention relates to a novel class of plant enzymes, thioesterases having preferential hydrolase activity toward one or more medium-chain acyl-ACP substrates. Purification, especially the removal of substantially all other plant proteins, and use of the plant thioesterase is provided, including the use of the protein as a tool in gene isolation for biotechnological applications and a description of various uses for such nucleic acid sequences thereto. The enzyme is preferrably obtained from Umbellularia californica and has preferential hydrolase activity toward C12 acyl-ACP substrate.
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Davies Huw M.
Pollard Michael R.
Calgene Inc.
Meller Michael V.
Naff David M.
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