Plant test procedure to detect natural, semi-synthetic,...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...

Reexamination Certificate

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Reexamination Certificate

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10319111

ABSTRACT:
The present invention relates to a method of testingBacopa monnieriresponse to abiotic stress factors and for testing bioactivity of natural, synthetic and semisynthetic compounds usingBacopa monnieriplant, which comprises growing the said plant and plant parts aseptically in MS 0 basal medium with agar in microcentrifuge tubes by adding the compounds to be tested either to the culture media or by spraying the said compounds on the plant or plant parts or on the said medium to detect the distinct morphological and cytological responses.

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Webster's Unabridged Dictionary of the English Language, 2001, Random House, New York, p. 364.
Thakur, et al; Department of Botany, University of Delhi/India; “In Vitro Shoot Bud Differentiation from Epidermal Cells of Stem Segments inBacopa monnieri(Linn.) Pennell” Beitrage zur Biologie der Pflanzen; 21 Beitr. Biol. Pflanzen 53, 321-330 (1977); Duncker & Humblot, Berlin.
D. Philcox; “Clarification of the nameBacopa monnieri(Scrophulariaceae)”; Kew Bulletin vol. 33(4); p. 679-680; provided by the University of Washington Libraries, US.
Gayoor, et al; “A Rapid Protocol for Micropropagation ofBacopa monniera(L.) Wettst.—an Important Medicinal Plant”; Plant Tissue Culture and Biotechnology, Dec. 1996, vol. 2, No. 4; p. 208-211; US.
Gayoor, et al; “Morphogenic Response and Isozymes andBacopa monniera(L.) Wettst Cultures Grown Under Salt Stress”; Phytomorphology, (1997) vol. 47, No. 1; pp. 97-106.
Gayoor, et al; “Morphogenic Response and Proline Content inBacopa monnieraCultures Grown under Copper Stress”; Plant Science 138 (1998) 191-195; Elsevier Science Ireland Ltd.
Ali, et al.; “Aluminium-Induced Morphogenic and Biochemical Variations ofBacopa monniera” J. Plant Biol. 41(3):240-245 (1998).
Tiwari, et al.; “Shoot Regeneration and Somatic Embryogenesis from Different Explants of Brahmi [Bacopa monniera(L.) Wettst.]”; Plant Cell Reports (1998) 17: 538-543.
Ali, et al; “Effect of Cadmium and Copper on Growth ofBacopa monnieraregenerants”; Biologia Plantarium 41 (4): 6356-639, 1998.
Biasi, L.A.; “Phytotoxicity of Three Antibiotics to Avocado Tissue Culture”; Bragantia, (1995) vol. 54, No. 2; pp. 251-256. 23 ref. ISSN: 0006-8705; Departmento de Horticultura, Brazil.

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