Plant, seeds and oil with increased saturated...

Organic compounds -- part of the class 532-570 series – Organic compounds – Fatty compounds having an acid moiety which contains the...

Reexamination Certificate

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C554S227000

Reexamination Certificate

active

07569712

ABSTRACT:
The invention relates to a sunflower seed, comprising sunflower oil having increased stearic acid content as compared to wild type seeds (preferably between 19.1 and 35% by weight related to the total amount of fatty acids in the oil and comprising in the oil at least 3.4% of the triacylglycerol species that have the general formula SMS and not more than 5-0% of triacylglycerol species that have the general formula SMM obtainable by treating parent seeds with a mutagenic agent during a period of time and in a concentration sufficient to induce one or more mutations in the genetic trait involved in stearic acid biosynthesis resulting in increased production of stearic acid, germinating the treated seeds and culturing progeny plants therefrom, collecting and analyzing progeny seeds, selecting seeds that have acquired the desirable genetic traits and optionally repeating the cycle of germination, culturing and collection of seeds. The invention further relates to oil extracted from the seeds, a method for preparing the sunflower seeds, a method for preparing such sunflower oil, sunflower plants produced from the seeds and use of the oil.

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Valle Fernandez-Moya, Enrique Martinez-Force and Rafael Garces.Iransge Identification of Triacylglycerol Species from High Saturated Sunflower(Helianthus annuus)Mutants. American Cancer Society (2000).
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R. Alvarez-Ortega, S. Cantisan, E. Martinez-Force, and R. Garces.Characterization of Polar and Nonpolar Seed Lipid Classes from highly Saturated Fatty Acid Sunflower Mutants. Lipids, vol. 32, No. 8, pp. 883-837 (1997).
N. Ruiz-Lopez, E. Martinez-Force, and R. Garces.Sequential one-step extraction and analysis of triacylglycerols and fatty aci s in plant tissues. Analytical Biochemistry 317, pp. 247-254 (2003).

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