Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide contains a tissue – organ – or cell...
Reexamination Certificate
2000-05-22
2002-04-23
McGarry, Sean (Department: 1635)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide contains a tissue, organ, or cell...
C536S023100, C536S023600, C536S024100, C435S006120, C435S320100, C435S410000, C800S295000
Reexamination Certificate
active
06376750
ABSTRACT:
BACKGROUND OF THE INVENTION
The use of transgenic plants for the production of pharmaceutical proteins and industrial enzymes has been proposed. In general, expression of recombinant proteins relies on stable integration of a heterologous gene into a host plant genome using, e.g., Agrobacterium-mediated. transformation or particle bombardment. In terms of cost, production of commercially valuable proteins using crops in the field is more competitive than other biological production systems (such as yeast, bacteria, or mammalian cell cultures) which require complex and high-maintenance bioreactors. Further, protein production in plant can be easily scaled-up to produce large quantities.
SUMMARY OF THE INVENTION
The invention is based on the discovery of a new promoter derived from a rice gene encoding for a glycine-rich RNA binding protein (designated OsGRP-A1). The OsGRP-A1 promoter can be used to express proteins in a plant and its tissues and organs, especially in the seedling, embryo, or sprout tissues. The sequence of OsGRP-A1 gene is shown below:
-1730
tagcttctaataattgttagtaggtatcaatagattgtttaatttaactg
-1680
gccatggaaagaatggtattggcatcaatggcatgaccgtttctataaaacccttcttat
-1620
tgatcaatgcatgatatctttaattaaatcccctttccctttttctcttctaaggtgatg
-1560
tttggaaccagatacttaactttagtctatatatttagacactaatttagagtattaaat
-1500
atagactacttacaaaactaattacataaatgaaagctaatttgcgagataaatttttta
-1440
agcctaattaatctataattagagaatttttactgtagcatcatataggcatatcatgga
-1380
ttaattaggctcaatagatttgtctcgcgaattagtccgagattatggatgagttttatt
-1320
gatagtctacgtttaatatttataattagtgtccaaacatcccatgtaatagggacttaa
-1260
aagttttagtcccatctaaacagggtctaagtccttctaaatctgttactcatataactg
-1200
tctaactgagataaagtttaaggttgtcatatcatatcatcgtcacgttatatatatgat
-1140
ccctgcacttctctttttatagaatggacgagactcttttttctgtatatgtagcggtct
-1080
tgtactcttgttagtaccattttgcgtcccattttgacgagacgactggcgtgccatttt
-1020
gcgtcctggttcattacagtctaatttggtgacaaacaaacaaggaacaaataggtccca
-960
tggtctagcggttaggacattggactctgaatccagtaacccgagttcaaatctcggtgg
-900
gaccttaattttctcggttttattttctgcctgagcttattgtcctcctcctgatttttt
-840
gttgttgtctattttctctgccggaaaaatgtatcaaactcgtcgattctactcgtttga
-780
gagcttactgtgatattgtccttctcctgaagtttctattttttactctctctgttatga
-720
aaattttcatgctagaatgatttacattgtgaaatggagagagaactcgtttgtgcttat
-660
ttatccttcccctgatttttttccacaccaaaacatatattgtgatggttgagtatgcta
-600
cgcgtctgacgtactacgagtttactccctccgtcccaaaaaaagacaaaccctgagttt
-540
tcatgtccaatgtttgatcatattatttgaaaaaattatgaaaaaattaaaaagccagtt
-480
acgtataaagtattaatcatattttatcatataacaacaatgaaaatactaattataaaa
-420
atttttcatataagacggacagttaaacgttggacacgaaaatctaggatttattttttt
-360
ttatagagggagtacgaggtaaaaatcgtcctcagcgccttcagaaaaaaaaaggacaaa
-300
aatcctcagcgccaaccgactccgctccacagaccacagccgcccaagtgtgcgaggaca
-240
acggcggcggcggcggcggctaggtttttgctgcacccgacgccaccgcccaccagcgag
-180
tgtggtgggccgcggcggcccataaaagaaatatctaggcggcccatgtagcgccagaaa
-120
atatcttctcccccgcctcgggatccttatcctccgcctcgcgcggggtgccgtccgatc
-60
agatcaggacggccgcgtggggc
tataaaa
ggagggggggtagggcaagcatgtcctcct
1
CGTGCTCTCTTTGAGGTGGGTTGGCTTCTCCTCCCCCTCTTTACCTTTTCCTCCTCGGTT
61
CGGTTCCGTGGTTCGTCTAGGGTTTAGTGGGTTGAGATGGCGGCGCCGGATGTCGAGTAC
M A A P D V E′Y
121
CGCTGCTTCGTCGGCGGCCTCGCCTGGGCCACCGACGACCGCTCCCTCGAGGCCGCCTTC
R C F V G G L A W A T D D R S L E A A F
181
TCCACCTACGGCGAGATCCTCGACTCCAAGgttcgccctcgctctcctacgccgtgtctt
S T Y G E I L D S K
241
gtgatgggttttttttgggtggtttctcgtgttggctggatctgtgttgaatttgtttgg
301
gtttttttgttggtttgctcggatctgtgatcctggggggttttgctcgtgctgttctga
361
tctcgttggtgccagatctgtgtggaggggttgatttggtggtttttttgggtggatctg
421
atggggatttgtgtgtttttgttttggttgtttgcagATCATCAACGACAGGGAGACGGG
I I N D R E T G
481
GAGGTCACGTGGGTTTGGCTTCGTCACCTTCTCCTCCGAGCAGTCGATGCGCGACGCCAT
R S R G F G F V T E S S E Q S M R D A I
541
CGAGGGCATGAACGGCAAGGAGCTCGACGGCCGCAACATCACCGTCAATGAGGCCCAGTC
E G M N G K E L D G R N I T V N E A Q S
601
CCGCCGCTCCGGCGGCGGAGGCGGGGGCTACGGCGGCGGCGGTGGCGGCTACGGCGGCGG
R R S G G G G G G Y G G G G G G Y G G G
661
TCGTGGAGGCGGCGGCTACGGAGGAGGTGGQGGCGGCGGCTACGGGCGCCGTGAGGGCGG
R G G G G Y G G G G G G G Y G R R E G G
721
CTACGGTGGCGGCGGCGGCTACGGCGGCGGCCGTGGCGGCGGCGGCGGCGGCTACGGTGG
Y G G G G G Y G G G R G G G G G G Y G G
781
CAGCCGTGGCGGCGGCTACGGCGGCGACTCCGGCGGGAACTGGAGGAACTGATTGGTGGG
S R G G G Y G G D S G G N W R N * (SEQ ID NO:4)
841
GCCCATCGTGGCCAGTTATCCTTAGCTATCCGTGTCAGAATCATCTTATCATCGAATCGA
901
GTCGTTATCGTGTCCAGTGGCTCTCTCGAGTCGAGAAGCCCTCTATCCATCCATCCAGTG
961
TTAGGTGTTCTTGCTCGGTGATCTTACCATGAATTGAGTTCGCTTTGGTTATGGTGTTTG
1021
AACTGCTTGTTGCTATCTATCGGAATGAAATGAAATAGAAAACAAGGAGAAAAAAAAGAG
1081
TTCGAAAGTTTTGTTCGCATACCATATATTTCCTTCCGGTGCGCGCTGTTTATTCCTCGC
1141
TCAGCAGCAAGATTGTTTGATCGATATTGCAGCAAGCAATTCACAATAAATATATTGCTA
1201
CACTCCTACTTCAAACTACACTGGTGGTCGGTGATTTTCAATAGCATGAACCTTAATTGA
1261
ACATCTGTGTAGCTTACATCTCCTTCGAAAGCTGCAATGCTTGAGAACTTGGAAAGAAAT
1321
tcttgtgatggcagaagctattcactgtccttcgctgcatttacagtccatacagacaca
1381
gcatttccattttgcacaagatagagaacaacaatcagccttttaggtcaatcccaagtg
1441
tgcatcttactgattgtcgaatatgtgctaagaacctgcaagagagtgaggatttttatc
1501
attgattgattgtcgaatatatgctaagaaccttcaagagagtgaggatttttatcattg
1561
atggagctttgtttctgtaatccaagatttgggaaacagtttatccatgttagggaaatg
1621
atttatgcaaacaatattttggagcaaaagttagatagtagtatatgtctgccgacgacc
1681
tcaacccaaaatattgttttgaggaactcttggattacagaacaaaatactgattccaaa
1741
caatcatacttttttaacacaaccatcacattggagatttcaaaacagcagagatgatag
1801
gatctactctttccatgtcttcatccaaacttaattaagctt (SEQ ID NO:3)
As indicated above, the complete nucleotide sequence of OsGRP-A1 is designated SEQ ID NO:3, while the complete amino acid sequence of the protein encoded by OsGRP-A1 is designated SEQ ID NO:4. The upper case nucleotides represent the cDNA sequence, while the lower case nucleotides represent the genomic sequences flanking the cDNA and in the intron. Nucleotide numberings are relative to the transcription start site. The putative TATA box is underlined. The OsGRP-A1 protein contains two consensus RNA binding domains (amino acids 10-15 and 49-56 of SEQ ID NO:4), whic
Chao Yu-Chan
Yu Su-May
Academia Sinica
Fish & Richardson P.C.
McGarry Sean
LandOfFree
Plant seedling and embryo promoter does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Plant seedling and embryo promoter, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Plant seedling and embryo promoter will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2919279