Plant seedling and embryo promoter

Details Plant seedling and embryo promoter Plant seedling and embryo promoter

2000-05-22

2002-04-23

McGarry, Sean (Department: 1635)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide contains a tissue, organ, or cell...

C536S023100, C536S023600, C536S024100, C435S006120, C435S320100, C435S410000, C800S295000

Reexamination Certificate

active

06376750

ABSTRACT:

BACKGROUND OF THE INVENTION
The use of transgenic plants for the production of pharmaceutical proteins and industrial enzymes has been proposed. In general, expression of recombinant proteins relies on stable integration of a heterologous gene into a host plant genome using, e.g., Agrobacterium-mediated. transformation or particle bombardment. In terms of cost, production of commercially valuable proteins using crops in the field is more competitive than other biological production systems (such as yeast, bacteria, or mammalian cell cultures) which require complex and high-maintenance bioreactors. Further, protein production in plant can be easily scaled-up to produce large quantities.
SUMMARY OF THE INVENTION
The invention is based on the discovery of a new promoter derived from a rice gene encoding for a glycine-rich RNA binding protein (designated OsGRP-A1). The OsGRP-A1 promoter can be used to express proteins in a plant and its tissues and organs, especially in the seedling, embryo, or sprout tissues. The sequence of OsGRP-A1 gene is shown below:
-1730
tagcttctaataattgttagtaggtatcaatagattgtttaatttaactg

-1680
gccatggaaagaatggtattggcatcaatggcatgaccgtttctataaaacccttcttat

-1620
tgatcaatgcatgatatctttaattaaatcccctttccctttttctcttctaaggtgatg

-1560
tttggaaccagatacttaactttagtctatatatttagacactaatttagagtattaaat

-1500
atagactacttacaaaactaattacataaatgaaagctaatttgcgagataaatttttta

-1440
agcctaattaatctataattagagaatttttactgtagcatcatataggcatatcatgga

-1380
ttaattaggctcaatagatttgtctcgcgaattagtccgagattatggatgagttttatt


-1320
gatagtctacgtttaatatttataattagtgtccaaacatcccatgtaatagggacttaa

-1260
aagttttagtcccatctaaacagggtctaagtccttctaaatctgttactcatataactg

-1200
tctaactgagataaagtttaaggttgtcatatcatatcatcgtcacgttatatatatgat

-1140
ccctgcacttctctttttatagaatggacgagactcttttttctgtatatgtagcggtct

-1080
tgtactcttgttagtaccattttgcgtcccattttgacgagacgactggcgtgccatttt

-1020
gcgtcctggttcattacagtctaatttggtgacaaacaaacaaggaacaaataggtccca

-960
tggtctagcggttaggacattggactctgaatccagtaacccgagttcaaatctcggtgg

-900
gaccttaattttctcggttttattttctgcctgagcttattgtcctcctcctgatttttt

-840
gttgttgtctattttctctgccggaaaaatgtatcaaactcgtcgattctactcgtttga

-780
gagcttactgtgatattgtccttctcctgaagtttctattttttactctctctgttatga

-720
aaattttcatgctagaatgatttacattgtgaaatggagagagaactcgtttgtgcttat

-660
ttatccttcccctgatttttttccacaccaaaacatatattgtgatggttgagtatgcta

-600
cgcgtctgacgtactacgagtttactccctccgtcccaaaaaaagacaaaccctgagttt

-540
tcatgtccaatgtttgatcatattatttgaaaaaattatgaaaaaattaaaaagccagtt

-480
acgtataaagtattaatcatattttatcatataacaacaatgaaaatactaattataaaa

-420
atttttcatataagacggacagttaaacgttggacacgaaaatctaggatttattttttt

-360
ttatagagggagtacgaggtaaaaatcgtcctcagcgccttcagaaaaaaaaaggacaaa

-300
aatcctcagcgccaaccgactccgctccacagaccacagccgcccaagtgtgcgaggaca

-240
acggcggcggcggcggcggctaggtttttgctgcacccgacgccaccgcccaccagcgag

-180
tgtggtgggccgcggcggcccataaaagaaatatctaggcggcccatgtagcgccagaaa

-120
atatcttctcccccgcctcgggatccttatcctccgcctcgcgcggggtgccgtccgatc

-60
agatcaggacggccgcgtggggc
tataaaa
ggagggggggtagggcaagcatgtcctcct

1
CGTGCTCTCTTTGAGGTGGGTTGGCTTCTCCTCCCCCTCTTTACCTTTTCCTCCTCGGTT

61
CGGTTCCGTGGTTCGTCTAGGGTTTAGTGGGTTGAGATGGCGGCGCCGGATGTCGAGTAC
                                    M A A P D V E′Y

121
CGCTGCTTCGTCGGCGGCCTCGCCTGGGCCACCGACGACCGCTCCCTCGAGGCCGCCTTC
R  C  F  V  G  G  L  A  W  A  T  D  D  R  S  L  E  A  A  F

181
TCCACCTACGGCGAGATCCTCGACTCCAAGgttcgccctcgctctcctacgccgtgtctt
S  T  Y  G  E  I  L  D  S  K

241
gtgatgggttttttttgggtggtttctcgtgttggctggatctgtgttgaatttgtttgg

301
gtttttttgttggtttgctcggatctgtgatcctggggggttttgctcgtgctgttctga

361
tctcgttggtgccagatctgtgtggaggggttgatttggtggtttttttgggtggatctg

421
atggggatttgtgtgtttttgttttggttgtttgcagATCATCAACGACAGGGAGACGGG
                                     I  I  N  D  R  E  T  G

481
GAGGTCACGTGGGTTTGGCTTCGTCACCTTCTCCTCCGAGCAGTCGATGCGCGACGCCAT
 R  S  R  G  F  G  F  V  T  E  S  S  E  Q  S  M  R  D  A  I

541
CGAGGGCATGAACGGCAAGGAGCTCGACGGCCGCAACATCACCGTCAATGAGGCCCAGTC
 E  G  M  N  G  K  E  L  D  G  R  N  I  T  V  N  E  A  Q  S

601
CCGCCGCTCCGGCGGCGGAGGCGGGGGCTACGGCGGCGGCGGTGGCGGCTACGGCGGCGG
 R  R  S  G  G  G  G  G  G  Y  G  G  G  G  G  G  Y  G  G  G

661
TCGTGGAGGCGGCGGCTACGGAGGAGGTGGQGGCGGCGGCTACGGGCGCCGTGAGGGCGG
 R  G  G  G  G  Y  G  G  G  G  G  G  G  Y  G  R  R  E  G  G

721
CTACGGTGGCGGCGGCGGCTACGGCGGCGGCCGTGGCGGCGGCGGCGGCGGCTACGGTGG
 Y  G  G  G  G  G  Y  G  G  G  R  G  G  G  G  G  G  Y  G  G

781
CAGCCGTGGCGGCGGCTACGGCGGCGACTCCGGCGGGAACTGGAGGAACTGATTGGTGGG
 S  R  G  G  G  Y  G  G  D  S  G  G  N  W  R  N  * (SEQ ID NO:4)

841
GCCCATCGTGGCCAGTTATCCTTAGCTATCCGTGTCAGAATCATCTTATCATCGAATCGA

901
GTCGTTATCGTGTCCAGTGGCTCTCTCGAGTCGAGAAGCCCTCTATCCATCCATCCAGTG

961
TTAGGTGTTCTTGCTCGGTGATCTTACCATGAATTGAGTTCGCTTTGGTTATGGTGTTTG

1021
AACTGCTTGTTGCTATCTATCGGAATGAAATGAAATAGAAAACAAGGAGAAAAAAAAGAG

1081
TTCGAAAGTTTTGTTCGCATACCATATATTTCCTTCCGGTGCGCGCTGTTTATTCCTCGC

1141
TCAGCAGCAAGATTGTTTGATCGATATTGCAGCAAGCAATTCACAATAAATATATTGCTA

1201
CACTCCTACTTCAAACTACACTGGTGGTCGGTGATTTTCAATAGCATGAACCTTAATTGA

1261
ACATCTGTGTAGCTTACATCTCCTTCGAAAGCTGCAATGCTTGAGAACTTGGAAAGAAAT

1321
tcttgtgatggcagaagctattcactgtccttcgctgcatttacagtccatacagacaca

1381
gcatttccattttgcacaagatagagaacaacaatcagccttttaggtcaatcccaagtg

1441
tgcatcttactgattgtcgaatatgtgctaagaacctgcaagagagtgaggatttttatc

1501
attgattgattgtcgaatatatgctaagaaccttcaagagagtgaggatttttatcattg

1561
atggagctttgtttctgtaatccaagatttgggaaacagtttatccatgttagggaaatg

1621
atttatgcaaacaatattttggagcaaaagttagatagtagtatatgtctgccgacgacc

1681
tcaacccaaaatattgttttgaggaactcttggattacagaacaaaatactgattccaaa

1741
caatcatacttttttaacacaaccatcacattggagatttcaaaacagcagagatgatag

1801
gatctactctttccatgtcttcatccaaacttaattaagctt (SEQ ID NO:3)
As indicated above, the complete nucleotide sequence of OsGRP-A1 is designated SEQ ID NO:3, while the complete amino acid sequence of the protein encoded by OsGRP-A1 is designated SEQ ID NO:4. The upper case nucleotides represent the cDNA sequence, while the lower case nucleotides represent the genomic sequences flanking the cDNA and in the intron. Nucleotide numberings are relative to the transcription start site. The putative TATA box is underlined. The OsGRP-A1 protein contains two consensus RNA binding domains (amino acids 10-15 and 49-56 of SEQ ID NO:4), whic

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