Plant rhamnogalacturonan II complexation of heavy metal cations

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Carbohydrate doai

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514 64, 536 2, 536123, 5361231, 436 79, 436 82, A61K 31715, C07H 100, G01N 3320

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active

058771646

ABSTRACT:
The present invention provides rhamnogalacturonan-II (RG-II) and relates to its ability to complex specific multivalent heavy metal cations. In the presence of boric acid, RG-II monomers form dimers that are cross-linked by a borate ester. The yield of such borate ester cross-linked dimers of RG-II is enhanced in the presence of specific heavy metal cations. The present invention further relates to the utility of RG-II in assays for the detection of specific heavy metal contamination; as a reagent useful in the removal of specific heavy metal cations contaminating foods and liquids, for example, fish, wines, etc.; as a pharmaceutical composition useful as an antidote in specific heavy metal cation poisoning; as a treatment for the detoxification of specific heavy metal cations from blood and/or tissues; and in a method of remediation of waters and soils contaminated with specific heavy metal cations.

REFERENCES:
Kobayashi et al. "Boron Nutrition of Cultured Tobacco BY-2 Cells. II. Characterization of the Borno-Polysaccharide Complex" Plant Cell Physiol. 38(6): 676-683 (1997).
Kobayashi et al. "Two Chains of Rhamnogalacturonan II Are Cross-Linked by Borate-Diol Ester Bonds in Higher Plant Cell Walls" Plant Physiol. 110:1017-1020 (1996).
O'Neill et al. J. Biol. Chem. Sep. 13, 1996, 271(37), 22923-30.
Matoh et al. Plant Cell Physiol. 1993, 34(4), 639-42.
Pellerin et al. Carbohydrate Research 1996, 290(2), 183-97.

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