Chemistry: molecular biology and microbiology – Apparatus – Differentiated tissue perfusion or preservation apparatus
Patent
1995-06-02
1997-01-28
Kepplinger, Esther
Chemistry: molecular biology and microbiology
Apparatus
Differentiated tissue perfusion or preservation apparatus
4352865, 4352891, 4352921, 4352941, 4352971, 4353051, 435809, 4352972, 4352975, 4353031, 4352866, 47 59, 47 60, 47 61, 47 62, 47 66, C12M 300, A01G 3102
Patent
active
055977316
ABSTRACT:
A plant propagation system and method are provided for promoting the growth of plant tissue into small plantlets. The plant propagation system includes sealed, semipermeable membrane vessels for completely enclosing plant material therein. The sealed vessels generally are translucent and permeable to gases and liquids while remaining impermeable to biological contaminates. Plant tissue originally extracted from a parent plant can be placed within the sealed vessels and grown heterotrophically. Once the plant material has developed the capability to photosynthesize, the sealed vessels can be transferred to a greenhouse environment for photoautotrophic growth. Once in a greenhouse environment, the sealed vessels are supported in trays and exposed to light, gases, water and a liquid nutrient solution for optimizing growth. A central controller can be included in order to automate the system for controlling the flow of fluids in and out of the vessel support trays while also monitoring system conditions. A disinfectant can also be circulated within the system for destroying biological contaminants in the tray and outside the sealed vessels for maintaining an aseptic environment.
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Adelberg Jeffrey W.
Young Roy E.
Cano Milton I.
Clemson University
Kepplinger Esther
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