Plant promoter comprising a G-box element, GCCACGTGCC or...

Details Plant promoter comprising a G-box element, GCCACGTGCC or... Plant promoter comprising a G-box element, GCCACGTGCC or...

1996-07-15

2001-02-13

Nelson, Amy J. (Department: 1649)

Multicellular living organisms and unmodified parts thereof and

Plant, seedling, plant seed, or plant part, per se

Higher plant, seedling, plant seed, or plant part

C432S120000, C432S120000, C536S024100

Reexamination Certificate

active

06187996

ABSTRACT:

FIELD OF INVENTION
The present invention relates to a plant promoter and an application thereof.
DESCRIPTION OF RELATED ART
For expression of an exogeneous structural gene of interest in a plant or plant cells, cauliflower mosaic virus 35S promoter (hereinafter referred to as “35S promoter”) which consists of about 0.8 kb has been used, but the minimal region of the 35S promoter (e.g., −90 region of the 35S promoter which consists of 98 nucleotide bases (−90 to +8)) has not been satisfactory for practical expression of a gene of interest because of the low transcription activity of the region (Odell et al., Nature 313: 810-812 (1985), Jensen et al., Nature 321: 669-674 (1986), Jefferson et al., EMBO J. 6: 3901-3907 (1987), Kay et al., Science 236: 1299-1302 (1987), Sanderset et al., Nucl. Acid. Res. 4: 1543-1558 (1987), etc.), Benfey et al., EMBO J. 8: 2195-2202 (1989).
SUMMARY OF INVENTION
To facilitate efficient expression of an exogeneous structural gene of interest in a plant or plant cells, the present inventors have extensively studied and found that a compact specific nucleotide sequence can enhance the expression of a gene of interest when connected upstream to a promoter including the minimal region as described above.
An object of the present invention is to provide a promoter functional in plant cells, which contains the nucleotide sequence shown in SEQ ID NO:1 or SEQ ID NO:2.
In addition to the specific nucleotides shown in SEQ ID NO:1 or SEQ ID NO:2, the promoter may include additional nucleotide on the 5′ and/or 3′ end of the sequence.
Another object of the present invention is to provide a plasmid comprising a promoter functional in plant cells which contains the nucleotide sequence shown in SEQ ID NO:1 or SEQ ID NO:2, and a terminator functional in plant cells.
An object of the present invention is to provide a plasmid comprising a promoter functional in plant cells which contains the nucleotide sequence shown in SEQ ID NO:1 or SEQ ID NO:2, a structural gene of interest and a terminator functional in plant cells.
A further object of the invention is to provide a plasmid pGbox10 shown in
FIG. 4
or pGbox11 shown in FIG.
5
.
Further objects of the present invention are to provide a plant or plant cell expressing a structural gene of interest under the control of the promoter described above, and a plant harboring the plasmid described above.
Still further objects of the present invention are to provide a method for expressing in plant cells a structural gene of interest, wherein the expression is controlled by the promoter described above, and a method for constructing a plasmid, which comprises connecting the promoter of the present invention, a structural gene of interest and a terminator functional in plant cells in this order.


REFERENCES:
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Salinas et al, The Plant Cell, vol. 4, pp. 1485-1493 (Dec. 1992).
Oeda et al, The EMBO Journal, vol. 10, No. 7, pp. 1793-1802 (1991).
Odell et al, Nature, vol. 313, pp. 810-812 (Feb. 28, 1985).
Otten et al, Nature, vol. 321, pp. 669-674 (Jun. 12, 1986).
Jefferson et al, The EMBO Journal, vol. 6, No. 13, pp. 3901-3907 (1987).
Kay et al, Science, vol. 236, pp. 1299-1302 (Jun. 5, 1987).
Sanders et al, Nucleic Acids Research, vol. 15, No. 4, pp. 1543-1558 (1987).
Benfey et al, The EMBO Journal, vol. 8, No. 8, pp. 2195-2202 (1989).
Ueda et al, The Plant Cell, vol. 1, pp. 217-227 (Feb. 1989).
Van de Rhee et al, The Plant Cell, vol. 2, pp. 357-366 (Apr. 1990).
Keil et al, The Plant Cell, vol. 2, pp. 61-70 (Jan. 1990).
M. E. Williams et al.,The Plant Cell,vol. 4, pp. 485-496 (Apr. 1992).
A. Galvanas et al., Genbank Database Accession No. L123533, XP002101289 (May 4, 1994).
R. Lafyatis et al.,Journal of Biological Chemistry,vol. 265, No. 31, pp. 19128-19136 (Nov. 5, 1990).
Genbank Database Accession No. E07891, XP002101288, 1997.

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