Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide contains a tissue – organ – or cell...
Patent
1997-09-30
2000-02-22
Smith, Lynette R. F.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide contains a tissue, organ, or cell...
536 241, 800278, 800295, 800313, 8003173, 8003174, 8003203, C12N 1529, C12N 504, A01H 400, A01H 500
Patent
active
060282509
DESCRIPTION:
BRIEF SUMMARY
FIELD OF THE INVENTION
The present invention relates to plant promoters which are useful for development of new plant varieties employing the gene recombination technology and the plant engineering such as functional modification etc. as well as is useful for the plant cell engineering such as functional modification of plant culture cells producing useful metabolites, DNA fragments in which useful genes are ligated to the promoters in such state that the useful genes can be expressed, and vectors containing the DNA fragments. Furthermore, the present invention relates to plants or plant cells that are transformed with the DNA fragments or vectors containing the DNA fragments, or to transgenic plants regenerated from the plant cells. Still furthermore, the present invention relates to a method for cloning the plant promoters.
PRIOR ART
Improvement of plants utilizing the gene engineering techniques has recently been using practically [Science, 244, 1293-1299 (1989)]. In particular, remarkable able progress has been made in the transformation system utilizing Ti plasmid and Ri plasmid that are contained in soil bacteria, Agrobacterium tumefaciens and Agrobacterium rhizogenes, whereby the system can be applicable not only to tobacco, Arabidopsis, and petunia that have been hitherto transformed but also to dicotyledonous plants such as azuki bean [Abstracts of Presentation at the Yeeting of NIHON SHOKUBUTU SOSHIKIBAIYOU GAKKAI (Japanese Association for Plant Tissue Culture), P. 124 (1990)] and to monocotyledonous plants such as rice [The Plant Journal, 6, 271-282 (1994)]. Moreover, for monocotyledonous plants whose representative example is the rice plant, a method comprising preparing a protoplast and then transferring a gene therein by electroporation has been practically used [Nature, 338, 274-276 (1989)]. In addition, there are many examples where genes are directly transferred into plants using the particle gun method [The Plant Journal, 2, 275-281 (1992)].
As for promoters which induce the tissue-specific expression of useful substances or enzymes, there have been heretofore isolated genes that express specifically in respective tissues of seed [SHOKUBUTU SAIBOU KOUGAKU (Plant Cell Technology), 3, 568-576 (1991)], respective tissues of leaves and flowers [Science, 250, 931-936 (1990)], tuber [SHOKUBUTU SAIBOU KOUGAKU (Plant Cell Technology), 3, 577-587 (1991)], tuberous root, and root nodule [Science, 250, 948-954 (1990)) and the expression by these promoters has been analyzed in transgenic plants.
However, most promoters that have been hitherto utilized for these vector systems are promoters originating from Ti plasmid contained in Agrobacterium tumefaciens and promoters originating from the genes of cauliflower mosaic virus (CaMV). These promoters constitutively express irrespective of growth stages and tissues of transgenic plants and can not be controlled. In addition, the expression level is low. Moreover, among promoters containing expression regulatory regions inducing the tissue-specific expression, none of the promoters induce the expression specifically at the site and the stage required for the reconstitution of plant cell wall xyloglucan.
Furthermore, in the field of plant cell engineering, even when one intends to produce a useful secondary metabolite in plant cells to be used for a plant tissue culture, there have been known many cases where the expression of an enzyme gene in a biosynthesis system of the metabolite is repressed due to the presence of a plant hormone essential for the cell growth, thereby repressing the production of the metabolite [Physiologia Plantarur, 80, 379-387 (1990)]. Therefore, it is extremely difficult to optimize the biosynthesis of a secondary metabolite by cells in the presence of a plant hormone necessary for the cell growth. Then, it is required to employ a two-stage culture method wherein the cell growth and the biosynthesis of a secondary metabolite are carried out under separate conditions [Nippon NOUGEIKAGAKU KAISHI (Journal of Agricultural Chemistry
REFERENCES:
patent: 4407956 (1983-10-01), Howell
patent: 5516694 (1996-05-01), Nishitani et al.
Kim et al. Plant Molecular Biology. vol. 24: 105-117, 1994.
Park et al. Plant J. 1996. vol. 9: 183-194.
Napoli et al. The Plant Cell. 1989. vol. 2: 278-289.
Giovannoni et al. The Plant Cell. 1989. vol. 1: 53-63.
Cornejo et al. Plant Molecular Biology. 1993. vol. 23: 567-581.
de Silva et al. Plant Journal. 1993. vol. 3: 701-711.
Wei Xu et al., "Arabidopsis TCH4, Regulated by Hormones and the Environment, Encodes a Xyloglucan Endotransglycosylase"; The Plant Cell, 7: 1555-1567, Oct. 1995.
Z. Schwarz-Sommer et al., "Genetic Control of Flower Development by Homeotic Genes in Antirrhinum Majus", Science, vol. 250, p. 931-936, Nov. 16, 1990.
J.P. Nap et al., "Development Biology of a Plant-Prokaryote Symbiosis: The Legume Root Nodule", Science, vol. 250, p. 948-954, Nov. 16, 1990.
D.M. Zurek et al., "Molecular Cloning and Characterization of a Brassinosteroid-Regulated Gene From Elongating Soybean (Glycine max L.) Epicotyls", Plant Physiology, vol. 104, p. 161-170, 1994.
J.I. Medford et al, "Molecular Cloning and Characterization of Genes Expressed in Shoot Apical Meristems", The Plant Cell, vol. 3, p. 359-370, Apr. 1991.
J. de Silva et al., "Molecular Characterization of a Xyloglucan-Specific Endo-(1.fwdarw.4)-.beta.-.sub.D -glucanase (Xyloglucan Endo-Transglycosylase) from Nasturtium Seeds", The Plant Journal, vol. 3, No. 5, p. 701-711, 1993.
K. Kato et al., "Liquid Suspension Culture of Tobacco Cells", Fermentation Technology Today, p. 689-695, 1972.
J. Gielen et al., "The Complete Nucleotide Sequence of the TL-DNA of the Agrobacterium Tumefaciens Plasmid pTiAch5", The EMBO Journal, vol. 3, No. 4, p. 835-846, 1984.
A. Depicker et al., "Nopaline Synthase: Transcript Mapping and DNA Sequence", Journal of Molecular and Applied Genetics, vol. 1, No. 6, p. 561-573, 1982.
A. Crossway et al., "Integration of Foreign DNA Following Microinjection of Tobacco Mesophyll Protoplasts", Molecular & General Genetics, vol. 202, p. 179-185, 1986.
F.A. Krens et al., "In Vitro Transformation of Plant Protoplasts with Ti-plasmid DNA", Nature, vol. 296, p. 72-74, Mar. 4, 1982.
T.M. Klein et al., "High-Velocity Microprojectiles for Delivering Nucleic Acids Into Living Cells", Nature, vol. 327, p. 70-73, May 7, 1987.
R.T. Fraley et al., "Liposome-Mediated Delivery of Tobacco Mosaic Virus RNA Into Tobacco Protoplasts: A Sensitive Assay for Monitoring Liposome-Proplast Interactions", Proceedings of the National Academy of Sciences of the U.S.A., vol. 79, p. 1859-1863, Mar. 1982.
M. Fromm et al., "Expression of Genes Transferred Into Monocot and Dicot Plant Cells by Electroporation", Proc. Natl. Acad. Sci. USA, vol. 82, p. 5824-5828, Sep., 1985.
A. de la Pena et al., "Transgenic Rye Plants Obtained by Injecting DNA Into Young Floral Tillers", Nature, vol. 325, p. 274-276, Jan. 15, 1987.
C.A. Rhodes et al., "Genetically Transformed Maize Plants From Protoplasts", Science, vol. 240, p. 204-207, Apr. 8, 1988.
K. Shimamoto et al., "Fertile Transgenic Rice Plants Regenerated From Transformed Protoplasts", Nature, vol. 338, p. 274-276, Mar. 16, 1989.
A. Hoekema et al., "A Binary Plant Vector Strategy Based on Seperation of vir-an T-region of the Agrobacterium Tumefaciens Ti-Plasmid", Nature, vol. 303, p. 179-180, May 12, 1983.
K. Skriver et al., "Cis-Acting DNA Elements Responsive to Gibberellin and its Antagonist Abscisic Acid", Proc. Natl. Acad. Sci. USA, vol. 88, p. 7266-7270, Aug., 1991.
S. Ohta et al., "Construction and Expression in Tobacco of a .beta.-Glucuronidase (GUS) Reporter Gene Containing an Intron Within the Coding Sequence", Plant Cell Physiol., vol. 31, No. 6, p. 805-813, 1990.
R.E. Sheehy et al., "Molecular Characterization of Tomato Fruit Polygalacturonase", Molecular & General Genetics, vol. 208, p. 30-36, 1987.
T. Minamikawa, "Regulation of the Maturation-Specific Expression of Seed Storage Protein Genes", Shokubutsu Saibo Kogaku, vol. 3, No. 7, p. 568-576, 1991.
K. Nakamura e
Anma Yoshiko
Asada Kiyozo
Kato Ikunoshin
Ohba Toshiharu
Takahashi Shuichi
Smith Lynette R. F.
Takara Shuzo Co, Ltd.
Zaghmout Ousama M-Fait
LandOfFree
Plant promoter and method for gene expression using said promote does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Plant promoter and method for gene expression using said promote, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Plant promoter and method for gene expression using said promote will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-522102