Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...
Reexamination Certificate
1999-07-13
2001-04-17
Fox, David T. (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
C800S287000, C800S298000, C435S069100, C435S419000, C435S468000, C435S252300, C435S320100, C536S024100, C536S025300, C536S023100, C536S023600
Reexamination Certificate
active
06218598
ABSTRACT:
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to a promoter functional in a plant cell.
2. Description of the Related Art
In order to express the desired gene in a host cell for altering traits of a host cell such as a plant cell, etc., the gene is placed under the control of a promoter functional in a host cell. When it is necessary to more highly express the desired gene in a specified tissue of a host than other tissues, a promoter conferring the higher transcription activity in the specified tissue than in the other tissues is used.
The promoter which has a longer DNA sequence is influenced by a variety of factors in a host cell or probability of recombination or deletion is increased when the promoter is introduced into a chromosome of a host cell. As a result, there arises a problem that the desired trait is not expressed when the promoter is introduced into a host cell.
Then, there has been a demand for a compact promoter which may be suitable for higher expression of the desired gene in the particular tissue than in the other tissues in a host.
DISCLOSURE OF THE INVENTION
Under these circumstances, the present inventors studied hard and, as a result, found the particular nucleotide sequence suitable for the above object, which resulted in completion of the present invention.
That is, the present invention provides:
1. a promoter which is characterized in that it has following DNA sequence (a) or (b) and a DNA having the nucleotide sequence shown by SEQ ID:No.1,
(a) a DNA sequence having the nucleotide sequence represented by at least nucleotide numbers 112-246 within the nucleotide sequence shown by SEQ ID:No.2,
(b) a DNA sequence having the nucleotide sequence represented by at least base numbers 186-282 within the nucleotide sequence shown by SEQ ID:No.3 (hereinafter referred to as the present promoter),
2. a promoter which is characterized in that it has the following DNA sequence (a) or
(b) and a DNA having the nucleotide sequence shown by SEQ ID:No.1,
(a) a DNA sequence represented by nucleotide numbers 112-246, nucleotide numbers 54-246 or nucleotide numbers 1-246 within the nucleotide sequence shown by SEQ ID:No.2,
(b) a DNA sequence represented by nucleotide numbers 186-282, nucleotide numbers 127-282 or nucleotide numbers 1-282 within the nucleotide sequence shown by SEQ ID:No.3,
3. the promoter according to 1 or 2, wherein a DNA having the nucleotide sequence shown by SEQ ID:No.1 is positioned 5′ upstream of the DNA sequence (a) or (b),
4. the promoter according to any one of 1-3, which is characterized in that it has two or more than two copies of the nucleotide sequence shown by SEQ ID:No.1,
5. the promoter according to claims
1
to
3
, which is characterized in that it has 4 or 8 copies of the nucleotide sequence shown by SEQ ID:No.1,
6. a chimera gene which is characterized in that it has the promoter of any one of 1-5 and a desired gene (hereinafter referred to as the present chimera gene),
7. a vector which is characterized in that it has the promoter of any one of 1-5 (hereinafter referred to as the present vector),
8. the vector according to 7, which is characterized in that it has a cloninig site and a terminator functional in a host cell, which is positioned 3′ downstream of the promoter,
9. a vector which is characterized in that it has the chimera gene of 6,
10. a transformant which is characterized in that the promoter of any one of 1-5, the chimera gene of 6 or the vector of any one of 7-9 is introduced into a host cell (hereinafter referred to as the present transformant),
11. the transformant according to 10, which is characterized in that the host cell is a microorganism,
12. the transformant according to 10, which is characterized in that the host cell is a plant cell,
13. a method for expressing gene, which is characterized by comprising a step of expressing the desired gene in a host cell under the control of the promoter of any one of 1-5 (hereinafter referred to as the present gene expression method),
14. use of the promoter of any one of 1-5 for controlling the expression of a desired gene in a host cell,
15. a method for producing a promoter, which is characterized by comprising a step of ligating a DNA having the following DNA sequence (a) or (b) and a DNA having the nucleotide sequence shown by SEQ ID:No.1 in an functional form in a host cell (hereinafter referred to as the present promoter manufacturing process),
(a) A DNA sequence having the nucleotide sequence represented by at least nucleotide numbers 112-246 within the nucleotide sequence shown by SEQ ID:No.2,
(b) A DNA sequence having the nucleotide sequence represented by at least nucleotide numbers 186-282 within the nucleotide sequence shown by SEQ ID:No.3,
16. a method for producing a promoter, which is characterized by comprising a step of ligating a DNA having the following DNA sequence (a) or (b) and a DNA having the nucleotide sequence shown by SEQ ID:No.1 in an functional form in a host cell,
(a) a DNA sequence represented by nucleotide numbers 112-246, nucleotide numbers 54-246 or nucleotide numbers 1-246 within the nucleotide sequence shown by SEQ ID:No.2,
(b) a DNA sequence represented by nucleotide numbers 186-282, nucleotide numbers 127-282 or nucleotide numbers 1-282 within the nucleotide sequence shown by SEQ ID:No.3.
Further scope of applicability of the present invention will become apparent from the detailed description given hereinafter. However, it should be understood that the detailed description and specific examples, while indicating preferred embodiments of the invention, are given by way of illustration only, since various changes and modifications within the spirit and scope of the invention will become apparent to those skilled in the art from this detailed description.
Throughout this specification and the claims which follow, unless the context requires otherwise, the word “comprise”, and variations such as “comprises” and “comprising”, will be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integer or step.
REFERENCES:
patent: 5959176 (1999-09-01), Torikai et al.
patent: 571741 A2 (1993-12-01), None
patent: 0571741A2 (1993-12-01), None
patent: 0754757A2 (1996-01-01), None
patent: 754757A2 (1997-01-01), None
patent: 0824150A2 (1998-02-01), None
Drew , R. L. K. Hort. Res. vol. 19, pp. 79-84, 1979.*
Ishige, Fumiharu et al., The Plant Journal, vol. 18, No. 4 (1999), pp. 443-448.
Ishige Fumiharu
Nishikawa Satomi
Oeda Kenji
Birch & Stewart Kolasch & Birch, LLP
Fox David T.
Ibrahim Medina A.
Sumitomo Chemical Company Limited
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