Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Patent
1996-01-26
1998-06-30
Guzo, David
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
4351723, 4353201, 435414, 435419, 536 231, 536 241, A01H 104
Patent
active
057736952
ABSTRACT:
A nuclear scaffold attachment region isolated from a tobacco gene, and a method of making recombinant cells having increased levels of expression of foreign genes therein, are described. The method comprises transforming the cell with a DNA construct comprising in the 5' to 3' direction, a transcription initiation region, a structural gene positioned downstream from the transcription initiation region and operatively associated therewith, and a scaffold attachment region of the nucleotide sequence provided herein, positioned either 5' to the transcription initiation region or 3' to the structural gene. DNA constructs and vectors employed in carrying out the foregoing method are also discussed.
REFERENCES:
patent: 5459252 (1995-10-01), Conkling et al.
G.C. Allen et al., Scaffold Attachment Regions Increase Reporter Gene Expression in Stably Transformed Plant Cells, Plant Cell 5:603-613 (1993).
G.C. Allen et al., Abstract, 4th International Congress of Plant Molecular Biology, Amsterdam (1994).
G.E. Hall et al., Abstract, Annual Meeting of the Society of Plant Physiologists, Portland, OR (1994).
M.A. Conkling et al., Isolation of Transcriptionally Regulated Root-specific Genes from Tobacco, Plant Physiol. 93:1203-1211 (1990).
G.E. Hall et al., Nuclear Scaffold and Scaffold-Attachment Regions in Higher Plants, Proc. Natl. Acad. Sci. 88:9320-9324 (1991).
G.E. Hall et al., Isolation and Characterization of Nuclear Scaffolds, In: Plant Molecular Biology (2d Ed.), Stanton, Gelvin and Schilperoort (Eds.); Kluwer Academic Publishers (1994).
Y.T. Yamamoto et al., Root-specific Genes from Tobacco and Arabidopsis Homologous to an Evolutionarily conserved Gene Family of Membrane Channel Proteins, Nucleic Acids Research 18:7449 (1990).
Y.T. Yamamoto et al., Characterization of cis-acting Sequences Regulating Root-specific Gene Expression in Tobacco, The Plant Cell 3:371-382 (1991).
Allen George C.
Hall, Jr. Gerald
Spiker Steven
Thompson William F.
Guzo David
North Carolina State University
Yurel Irem
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