Plant methionine synthase gene and methods for increasing...

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...

Reexamination Certificate

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C435S320100, C435S468000, C435S410000, C435S419000, C435S069100, C435S183000, C536S023100, C536S023200, C536S023400, C536S023600, C536S024100, C800S281000, C800S290000, C800S295000, C800S298000

Reexamination Certificate

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07026527

ABSTRACT:
This invention relates to a nucleic acid fragment encoding a plant 5-methyltetra-hydropteroyltriglutamate-homocysteine methyltransferase or methionine synthase. The invention also includes chimeric genes, a first encoding a plant methionine synthase (MS) gene, a second encoding a plant cystathionine γ-synthase (CS) gene, a third encoding feedback-insensitive aspartokinase (AK) or bifunctional feedback-insensitive aspartokinase-homoserine dehydrogenase (AK-HDH), which is operably linked to a plant chloroplast transit sequence, and a fourth encoding a methionine-rich protein, all operably linked to plant seed-specific regulatory sequences. Methods for their use to produce increased levels of methionine in the seeds of transformed plants are provided.

REFERENCES:
patent: 5912414 (1999-06-01), Falco et al.
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Madison, James T. et al., Characterization of soybean tissue culture cell lines resistant to methionine analogs,Plant Cell Reports, 7, 473-476, 1988.
Giovanelli, John et al., In Vivo Regulation of De Novo Methionine Biosynthesis in a Higher Plant (Lemna),Plant Physiol., 77, 450-455, 1985.
Eichel, Johannes et al., Vitamin-B12-independent methionine synthase from a higher plant (Catharanthus roseus)—Molecular characterization, regulation, heterologous expression, and enzyme properties,Eur. J. Biochem., 230, 1053-1058, 1995.
Peterson, Maike et al., Plant Gene Register PGR95-049—Full-length cDNA clone fromColeus blumei(Accession No. Z49150) with high similarity to cobalamine-indepdendent methionine synthease (1),Plant Physiol., 109, 338, 1995.
Ravanel, Stephane et al., The specific features of methionine biosynthesis and metabolism in plants,Proc. Natl. Acad. Sci. USA, 95, 7805-7812, Jun. 1998.
Yamada, Yazuhiro et al., Cloning, Sequencing, and Heterologous Expression of Rat Methionine Synthase cDNA,Biosci. Biotechnol. Biochem, 62(11): 2155-2160, 1998.
Thompson, Gregory A. et al., Methionine Biosynthesis inLemna, Plant Physiol., 69, 1077-1083, 1982.
Le Guen, L. et al., Gene density and organization in a small region of theArabidopsis thalianagenome,Mol. Gen. Genet., 245, 390-396, 1994.

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