Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Reexamination Certificate
2008-03-11
2008-03-11
Davis, Ruth A (Department: 1651)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
C435S157000, C435S158000, C435S162000, C435S171000
Reexamination Certificate
active
11364029
ABSTRACT:
The present invention relates to a plant mangrove-associated fungusCurvularia lunata, and a simple and efficient method of obtaining high yield of pure mannitol from a plant mangrove-associated fungusCurvularia lunata, said method comprising steps of cutting the leaves into small pieces and placing it on potato dextrose agar (PDA) plates for 48 hours, maintaining the culture at temperature ranging between 26-47° C. and salinity ranging between 4-32 ppt with occasional stress conditions for about 17-19 days to obtain mycelial mat, sonicating the mat to lyse the cells, extracting crude from the sonicated mat using methanol repeatedly, concentrating the crude, treating the concentrated crude extract with solvents of increasing polarity, obtaining aqueous fraction as white powder residue after the treatment containing crude mannitol, purifying the crude mannitol by chromatography to obtain pure mannitol with about 75% of the total crude extract.
REFERENCES:
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Weymarn et al., “Production of D-Mannitol by Heterofermentative Lactic Acid Bacteria”, Process Biochemistry vol. 37:1207-1213, (2002).
Domelsmith et al., “Production of Mannitol By Fungi From Cotton Dust”, Applied and Environmental Microbiology, vol. 54(7):1784-1790, (1988).
Yun et al., “A Comparative Study of Mannitol Production by Two Lactic Acid Bacteria”, Journal of Fermentation and Bioengineering, vol. 85(2):203-208, (1998).
Yun et al., “Mannitol Accumulation During Fermentation of Kimchi”, Journal of Fermentation and Bioengineering, vol. 81(3):279-280, (1996).
Paraszkiewicz et al., “Emulsifier production by steroid transforming filamentous fungusCurvularia lunata. Growth and product characteristics”, J. of Biotechnology, Jan. 2002 (92) 287-294.
Collins et al., “Biotransformation of cadina-4, 10(15)-dien—3—one and 3-alpha-hydroxycadina-4, 10(5)-diene byCurvularia lunataATCC 12017”, Phytochemistry, Mar. 2002 (59) 489-492.
D'Souza Lisette
Devi Prabha
Naik Chandrakant Govind
Peketi Asha
Rodrigues Ely
Council of Scientific and Industrial Research
Davis Ruth A
Hobbs Ann S.
Venable LLP
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