Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Plant cell or cell line – per se – contains exogenous or...
Patent
1994-04-21
2000-07-25
Prouty, Rebecca E.
Chemistry: molecular biology and microbiology
Plant cell or cell line, per se ; composition thereof;...
Plant cell or cell line, per se, contains exogenous or...
4351723, 435193, 4352523, 4353201, 435325, 435 912, 536 231, 536 232, 536 236, 536 243, 536 241, 536 2433, C12N 1554, C12N 910, C12N 510, C12N 1563
Patent
active
060935685
ABSTRACT:
This invention relates to plant LPAATs, means to identify such proteins, amino acid and nucleic acid sequences associated with such protein, and methods to obtain, make and/or use such plant LPAATs. Purification, especially the removal of plant membranes and the substantial separation away from other plant proteins, and use of the plant LPAAT is provided, including the use of the protein as a tool in gene isolation for biotechnological applications. In addition, nucleic acid sequences encoding LPAAT protein regions are provided, and uses of such sequences for isolation of LPAAT genes from plants are considered.
REFERENCES:
Bafor, et. al., "Substrate Specificities of Glycerol Acylating Enzymes from Developing Embryos of Two Cuphea Species", Physiochemistry 1992 vol. 31, No. 9 2973-2976 *08/224,625.
Bafor, et. al., "Regulation of Triacylglycerol Biosynthesis in Embryos and Microsomal Preparations from the Developing Seeds of Cuphea lanceolata" Biochem J. 1990 vol. 272 31-38 *08/224,625.
Bafor, et. al., Properties of Glycerol Acylating Enzymes in Microsomal Preparations from the Developing Seeds of Safflower (Carthamus tinctorius) and Turnip Rape (Brassica campestris) and their Ability to Assemble Cocoa-Butter Type Fats JAOCS 1990 vol. 67, No. 4 217-225 *08/224,625.
Bernerth, et. al., "Utilization of Erucoyl-CoA by Acyltransferases from Developing Seeds of Brassica napus (L.) Involved in Triacylglycerol Biosynthesis" Plant Science 1990 vol. 67 21-28 *08/224,625.
Cao, et. al., "Lysophosphatidate Acyltransferase in the Microsomes from Maturing Seeds of Meadowfoam (Limnanthes alba)" Plant Physiol. 1990 vol. 94 1199-1208 *08/224,625.
Coleman, J., "Characterization of the Escherichia coli Gene for 1-acyl-sn-glycerol-3-phosphate Acyltransferase (plsC)", Mol. Gen. Genet. 1992 vol. 232 295-303 *08/224,625.
Edwards, et. al. , "Partial Purification and Properties of A Microsomal Lysophosphatidic Acid Acyltransferase from Oilseed Rape", Biochemical Society Transactions 1989 vol. 17 684-685 *08/224,625.
Frentzen, et. al., "Specificaties and Selectivities of Glycerol-3 Phosphate Acyltransferase and Monoacylglycerol-3-Phosphate Acyltransferase from Pea and Spinach Chloroplasts", Eur. J. Biochem 1983 vol. 129 629-636 *08/224,625.
Frentzen, et. al., "Intraorganelle Localization and Substrate Specificities of the Mitochondrial Acyl-CoA: sn-Glycerol-3-Phosphate O-Acyltransferases and Acyl-CoA: 1-Acyl-sn-Glycerol-3-Phosphate O-Acyltransferase from Potato Tubers and Pea Leaves" Eur. J. Biochem 1990 vol. 187 395-402 *08/224,625.
Hares, et. al., "Substrate Specificities of the Membrane-Bound and Partially Purified Microsomal Acyl-CoA:1-Acylglycerol-3-Phosphate Acyltransferase from Etiolated Shoots of Pisum satavium (L.)" Planta 1991 vol. 185 124-131 *08/224,625.
Hares, et. al., "Properties of the Microsomal Acyl-CoA: sn-1-Acyl-Glycerol-3-Phosphate Acyltransferase from Spinach (Spinacia oleracea L.) Leaves" J. Plant Physiol 1987 vol. 131 49-59 *08/224,625.
Ichihara, et. al., "1-Acyl-sn-Glycerol-3-Phosphate Acyltransferase in Maturing Safflower Seeds and its Contributions to the Non-Random Fatty Acid Distribution of Triacylglycerol" Eur. J. Biochem 1987, vol. 167 339-347 *08/224,625.
Laurent, et. al. "Organ- and Development-Specific Acyl Coenzyme A Lysophosphatidate Acyltransferase in Palm and Meadowfoam" Plant Physiol. 1992 vol. 99 1711-1715 *08/224,625.
Nagiec, et. al. "A Suppressor Gene that Enables Saccharomyces cerevisiae to Grow without Making Sphingolipids Encodes a Protein that Resembles an Escherichia coli Fatty Acyltransferase" The Journal of Biological Chemistry 1993, vol. 268 No. 29 22156-22163 *08/224,625.
Oo, et. al. "Lysophosphatidate Acyltransferase Activities in the Microsomes from Palm Endosperm, Maize Scutellum, and Rapeseed Cotyledon of Maturing Seeds" Plant Physiol. 1989, vol. 91 1288-1295 *08/224,625.
Rajasekharan, et. al., "A Direct Nonchromatographic Assay for 1-Acyl-sn-Glycerol-3-Phosphate Acyltransferases" Analytical Biochemistry 1988, vol. 173 376-382 *08/224,625.
Slabas, et. al. "Plastid and Cytoplasmic Synthesis of Lipids" Biochemistry and Molecular Biology of Membrane and Storage Lipids of Plants, Edited by Norio Murata, Chris Somerville, Proceedings US/Japan Binational Seminar, Kona, Hawaii Dec. 13-17, 1992 113-120 Enclosed.
Slabas, et. al. "Fatty Acid Synthesis in Oilseeds" Proceedings of the Phytochemical Society of Europe Chapter 5, "Seed Storage Compounds" Edited by Shewry, Peter and Stobart, Keith Clarendon Press 1993, 87-95 *08/224,625.
Sun, et. al. "Acyl Coenzyme A Preference of the Glycerol Phosphate Pathway in the Microsomes from the Maturing Seeds of Palm, Maize and Rapeseed" Plant Physiol. 1988 vol. 88 56-60 *08/224,625.
Taylor, et. al., "Biosynthesis of Triacylglycerols in Brassica napus L. cv. Reston; Target: Trierucin" National Research Council of Canada No. 35122, Chapter 10 77-102 *08/224,625.
Wolter, et. al. "Biochemische und Molekularbiologische Ansatze zur Veranderung der Fettsaurezusammensetzung des Rapsols" Fat. Sci. Technol. 1991 vol. 93 No. 8 288-290 *08/224,625.
S.J. Gould et al., "Use of the DNA Polymerase Chain Reaction for Homology Probing . . . ", Proc. Natl. Acad. Sci., 86: 1934-1938 (Mar. 1989).
B.G. Lyapkov et al., "Molecular Forms of Triacylglycerols From Edible Vegetable Oils", Prikl. Biokhim. Mikrobiol. 26(2) 157-167 (1990).
D.L. Cooper et al., "Full Length cDNA Cloning Utilizing the Polymerase Chain Reaction, a Degenerate Oligonucleotide Sequence and a Universal Primer", Biotechniques 9(1) 60-65, 1990.
M.A. Frohman et al., "Rapid Production of Full-Length cDNAs From Rare Transcripts: Amplification Using a Single Gene-Specific Oligonucleotide Primer", Proc. Natl. Acad. Sci. 85: 8998-9002, Dec. 1988.
N. Zilberberg et al., "Raid Isolation of Full Length cDNA Clones by Inverse PCR: Purification of a Scorpion cDNA Family Encoding Alpha-Neurotoxins", Anal. Biochem. 209: 203-205, 1993.
G. Peterek et al., "Heterologous Complementation For Cloning Genes Encoding Microsomal 1-Acylglycerol-3-Phosphate Acyltransferase", Biol. Chem. Hoppe-Seyler 374(8): 533, Aug. 1993.
A.P. Brown et al., "Identification of a cDNA That Encodes a 1-Acyl-sn-Glycerol-3-Phosphate Acyltransferase From Limnanthes douglasii", Plant Mol. Biol. 29: 267-278, 1995.
GenBank entry Z46846, 1994.
Davies Huw Maelor
Hawkins Deborah
Nelsen Janet
Calgene Inc.
Prouty Rebecca E.
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