Plant isoflavonoid hydroxylases and methods of use thereof

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide confers pathogen or pest resistance

Reexamination Certificate

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C800S298000, C800S287000, C800S320000, C800S312000, C536S023100, C536S023200, C536S024100, C435S419000

Reexamination Certificate

active

07442851

ABSTRACT:
The invention provides plant isoflavonoid hydroxylase coding sequences. Also provided are constructs comprising these sequences, plants transformed therewith and methods of use thereof. In certain aspects of the invention, plants transformed with the nucleic acids are provided exhibiting improved pest and disease resistance. Plants provided by the invention may also exhibit improved nutritional qualities.

REFERENCES:
patent: 2004/0093632 (2004-05-01), Dixon et al.
patent: 2006/0123508 (2006-06-01), Dixon et al.
patent: WO 00/37652 (2000-06-01), None
patent: WO 02/066625 (2002-08-01), None
patent: WO 03/031622 (2003-04-01), None
patent: WO 03/040306 (2003-05-01), None
patent: WO 03/093464 (2003-11-01), None
patent: WO 2004/090136 (2004-10-01), None
Liu C. et al. PNAS, 2002, vol. 99, No. 22; pp. 14578-14583.
Akashi et al., “CYP81E1, a Cytochrome P450 cDna of Licorice (Glycyrrhiza echinata L.) Encodes isoflavone 2′-hydroxylase,”Biochemical and Biophysical Research Communications, 251:67-70, 1998.
Bhandari et al., “Biosynthesis of the A/B/C/D- ring system of the rotenoid amorphigenin byAmorpha fruticosaseedlings,”J. Chem. Soc. Perkin Trans., 839-849, 1992.
Choudhary et al., “Stress responses in alfalfa (Medicago sativa L.) IV. Expression of defense gene constructs in electroporated suspension cell photoplasts,”Plant Cell Rep., 9:42-46, 1990.
Clemens and Barz, “Cytochrome P450- dependant methylenedioxy bridge formation in Cicer Arietinum,”Phytochemistry, 41:457-460, 1996.
Clemens et al., “Characterization of Cytochrome P450-dependent isoflavone hydroxylasas from chickpea,”Phytochemistry, 32:653-657, 1993.
Dewick, “Isoflavonoids,”The Flavonoids: Advances in Research Since 1986., Harborne (Ed.), London: Chapman and Hall, 117-238, 1993.
Dixon and Ferreira, “Molecules of Interest Genistein,”Phytochemistry, 60:205-211, 2002.
Dixon and Sumner, “Legume natural products: understanding and manipulating complex pathways for human and animal health,”Plant Physiol., 131:878-885, 2003.
Dixon et al., “The phenylpropanoid pathway and plant defence-a genomics perspective,”Mol. Plant Pathol., 3:371-390, 2002.
Dixon, “Isoflavonoids: biochemistry, molecular biology, and biological functions,”Comprehensive Natural Products Chemistry, Barton et al. (ed), Elsevier, 1:773-823, 1999.
GenBank Accession No. AB001379, Apr. 21, 2000.
GenBank Accession No. AB022732, Apr. 21, 2000.
GenBank Accession No. AB025016, Jul. 19, 2003.
GenBank Accession No. AJ012581, May 1, 1999.
GenBank Accession No. AJ238439, Oct. 22, 1999.
GenBank Accession No. AY166658, Apr. 20, 2004.
GenBank Accession No. AY278227, Nov. 21, 2003.
GenBank Accession No. AY278229, Nov. 21, 2003.
Gunia et al., “Elicitor induction of cytochrome P450 monooxygenases in cell suspension cultures of chickpea (Cicer arietinum L.) and their involvement in pterocarpan phytoalexin biosynthesis,”Z. Naturforsch: 46:58-66, 1991.
Hahn et al., “ESTs from roots of Medicago truncatula treated with oligogalacturonides of DP 6-20,” 2001, Accession #BG648057..
He and Dixon, “Genetic manipulation of isoflavone 7-O-methyltransferase enhances biosynthesis of 4′0-methylated isoflavonoid phytoalexins and disease resistance in alfalfa,”Plant Cell, 12:1689-1702, 2000.
Hinderer et al., “Microsomal isoflavone 2′-and 3′-hydroxylases from chickpea (Cicer arietinum L.) cell suspensions induced for pterocarpan phytoalexin formation,”Febs Letters, 214:101-106, 1987.
Kochs and Grisebach, “Phytoalexin synthesis in soybean: purification and reconstitution of cytochrome P450 3,9-dihydroxypterocarpan 6a-hydroxylase and separation from Cytochrome P450 cinnamate 4-hydroxylase,”Arch. Biochem. Biophys., 273:543-553, 1989.
Lambert et al., “Production of rotenoids by heterotrophic and photomixotrophic cell cultures of tephrosia vogelii,”Phytochemistry, 34:1515-1520, 1993.
LatundeDada et al., “Flavonoid 6-hydroxylase from soybean (Glycine max. L.), a novel plant P-450 monooxygenase,”J. Biol. Chem., 276:1688-1695, 2001.
Liu and Dixon, “Elicitor-induced association of isoflavone O-methyltransferase with endomembranes prevents the formation and 7-O-methylation of daidzein during isoflavonoid phytoalexin biosynthesis,”Plant Cell, 13:2643-2658, 2001.
Liu et al., “Bottlenecks for metabolic engineering of isoflavone glycocojugates in Arabidopsis,”Proc. Natl. Acad. Sci USA, 99:14578-14583, 2002.
Liu et al., “Regiospecific hydroxylation of isoflavones by ctyochrome P450 81E enzymes from medicago truncatula,”The Plant Journal, 36:471-484, 2000.
Mackenbrock and Barz, “3′-Hydroxylation of 4′-Methoxyisoflavones by Fusarium oxysporum f. lycopersici,”Z. Naturforsch. 38:708-710, 1983.
Mackenbrock et al., “Accumulation and metabolism of medicarpin and maackiain maolnylgucosides in elicited chickpea (Cicer arietinum L.) cell suspension cultures,”J. Plant Physiol., 142:385-391. 1993.
Mihaliak et al., “Cytochrome P450 terpene hydroxylases,”Meth. Plant Biochem., 9:261-279, 1993.
Nakamura et al., “Induction of isoflavonoid and retrochalcone branches of the flavonoid pathway in culturedGlycyrrhiza echinatacells treated with yeast extract,”Biosci. Biotechnol. Biochem., 63:1618-1620, 1999.
Overkamp and Barz, “Cloning of two cicer arietinum L. cDNA's encoding Cytochrome P450's highly homologous Isoflavone 2′-Hydroxylase from licorice,”Plant Physiol., 120:935, 1999.
Overkamp et al., Cloning and characterization of eight cytochrome P450 cDNAs from chickpea (Cicer arietinum L.) cell suspension cultures,Plant Science, 155:101-108, 2000.
Pompon et al., “Yeast expression of animal and plant P450s in optimized redox environments,”Meth. Enzymol., 272:51-64, 1996.
Shimada et al., “Induction of isoflavonoid pathway in the model legumeLotus japonicus: molecular characterization of enzymes involved in phytoalexin biosynthesis,”Plant Science, 160:37-47, 2000.
Simmonds and Stevenson, “Effects of isoflavonoids fromCiceron larve ofHeliocoverpa armigera,”J. Chem. Ecol., 27:965-977, 2001.
Tebayashi et al., “Elicitor-induced changes in isoflavonoid metabolism in red clover roots,”J. Exp. Bot., 52:681-668, 2001.
Tolleson et al., “Metabolism of Biochanin A and formononetin by human liver microsomes in vitro,”J. Agric. Food Chem., 50:4783-4790, 2002.

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