Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;...
Reexamination Certificate
1999-08-16
2003-02-04
Moezie, F. T. (Department: 1653)
Chemistry: molecular biology and microbiology
Plant cell or cell line, per se ; composition thereof;...
C435S431000, C530S330000, C530S344000
Reexamination Certificate
active
06514759
ABSTRACT:
FIELD OF THE INVENTION
The present invention relates to peptides having the properties of a plant growth factor.
BACKGROUND OF THE INVENTION
The following plant-derived plant growth factors are known: barley-derived, fat-soluble fatty acid having a molecular weight of 600 or less (Journal of Plant Physiology, Vol. 121, pp. 181-191, 1985); pine-derived growth factor consisting of oligosaccharides having a molecular weight of 1000 or less (Plant Cell, Tissue and Organ Culture, Vol. 26, pp. 53-59, 1991); carrot-derived, heat-stable growth factor having a molecular weight of about 700 (Plant Science, Vol. 51, pp. 83-91, 1987); and black Mexican maize-derived growth factor which has a molecular weight of 1350 or less, oligosaccharide-like characteristics, and is not adsorbed to either of anion-exchange resin or cation-exchange resin in a buffer at pH of 5 (Journal of Plant Physiology, Vol. 132, pp. 316-321, 1988).
It is difficult to isolate and purify such known plant-derived plant growth factors, and there is no known technique for mass-production of these factors. In order to effectively use a plant growth factor as a plant cell growth promoter, it must be mass-producible.
SUMMARY OF THE INVENTION
The present invention relates to peptides having the properties of a plant growth factor and of the formula
wherein R
1
and R
2
are the same or different and each represents SO
3
H or H; X represents an &agr;-amino acid or a single bond; Z
1
and Z
2
are the same or different and each represents an &agr;-amino acid; and Y represents OH or NH
2
. This peptide has plant growth factor properties.
REFERENCES:
patent: 0 220 379 (1987-05-01), None
patent: 0 448 093 (1991-09-01), None
patent: WO 94/11018 (1994-05-01), None
File Scisearch on STN. An. No. 93:102878. ‘Effects of Elevated Sucrose Phosphate Synthase Activity on Photosynthesis, Assimilate Partitioning, and Growth in Tomato (Lycoersicon-Esculentumvar UC82B)’ Plant Physiology, Feb. 1993, vol. 101, No. 2, pp. 535.*
Somers et al. ‘The Effects of Contitioned Medium on Clony Formation From ‘Black Mexican Sweet’ Corn Protoplasts’, Plant Science, 1983. vol. 53. pp. 249-256.*
Barlos et al. Application of 2-chlorotrityl resin in solid phase synthesis of (Leu15)-gastrin I and unsulfated Cholecystokinin octapeptide, International Journal of Peptide and Protein Research 38:555-561 (Dec. 1991).
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Lou, et al.,Structure-activity relationship of a novel peptide substrate for p60c-src protein tyrosine kinase, Lett. Pept. Sci. 2(5):289 (1996); Chem. Abstr. No. 1996:86343.
Matsubayashi, et al.,Phytosulfokine, sulfated peptides that induce the proliferation of single mesophyll cells of Asparagus officinalis, L. P.N.A.C. USA, 93(15):7623-7627 (1996).
Novak, et al., Chem. Abstr. No. 1975:150793.
Novak, et al.,Tyrosyl Peptide Models for Acidic Protein-DNA Interactions, Nature New Biology 243:155-157 (1973).
Teasdale and Richards,Study of a factor produced by suspension-cultured Pinus radiata cells which enhances cell growth at low inoculum densities, Plant Cell, Tissue and Organ Culture, 26:53-59 (1991).
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Matsubayashi Yoshikatsu
Sakagami Youji
Gupta Anish
Kyowa Hakko Kogyo Co. Ltd.
Moezie F. T.
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