Plant generation method

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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4352405, 43524054, C12N 500

Patent

active

048409065

ABSTRACT:
The invention provides a method of generating barley plants comprising culturing barley microspores on a sugar-containing medium and harvesting green plantlets from the medium characterized in that the sugar in the sugar-containing medium is at least one sugar selected from sucrose; glucose; and oligosaccharides and polysaccharides containing at least two glucose residues; wherein the concentration of sucrose and glucose in the medium is not greater than 0.03 mol/l; and a method of generating barley seed which comprises the additional steps of cultivating green plantlets and deriving seed from resulting mature plants.

REFERENCES:
Grant et al., 1987, pp. 409, 463 In: Grant & Hackh's Chemical Dictionary, 5th Edition, (Grant et al., eds.), McGraw-Hill: New York.
Kirk-Othmer, 1964, Encyclopedia of Chemical Technology (John Wiley and Sons), p. 133.
Gonzalez-Medina et al., 1978, Euphytica 27(2): 553-559.
Raquin, Z. Pflanzenphysial Bd., 111, S, 453-457 (1983).
Snape, J. W., Induced Variability in Plant Breeding, PUDOC, Wageningen (ISBN 90 220 07960), 1982, pp. 52-58.
Huang et al., Ann. Bot., 49, pp. 77-88 (1982).
Hunter, Plant Cell Reports, 1985, 4, pp. 267-268.

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