Plant gene construct comprising male flower specific promoters

Details Plant gene construct comprising male flower specific promoters Plant gene construct comprising male flower specific promoters

1994-08-22

1998-09-15

Benzion, Gene

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 235, 536 236, 536 237, 47 58, 47DIG1, C12N 1511, C12N 1582, A01H 100

Patent

active

058080341

ABSTRACT:
Male sterility is imparted to a plant by a cascade of gene sequences which expresses a protein which disrupts the biosynthesis of viable pollen. Expression of the disrupter protein is restricted to male parts of the plant by an upstream promoter sequence which is specific to male flowers, the male specific promoter being under control of an operator sequence. The cascade also includes a gene encoding a repressor protein specific for that operator. Expression of the repressor protein is under control of a chemically inducible promoter which is inducible by the application to the plant by, spraying or like process, of an exogenous chemical. In the absence of the exogenous chemical inducer, no repressor protein is expressed, resulting in expression of the disrupter protein and, consequently, male sterility. Fertility may be restored to the plant, when required for maintenance of the line, by spraying with the inducer, resulting in expression of the repressor which binds the operator and inhibits expression of the disrupter protein.

REFERENCES:
Bouillaud et al (1985) Biochem. Biophysic. Res. Comm. vol. 157 #2 pp. 783-792.
Figge et al (1988) Cell vol. 52, pp. 713-722.
Stinson et al (1987) Plant Physiology vol. 83 pp. 442-447.
Eckes et al (1986) Mol. Gen. Genet. vol. 205 pp.14-22.
Wiegard et al (1986) Plant Mol. Biol. vol. 7, pp. 235-243.

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