Plant fatty acid desaturases and alleles therefor

Details Plant fatty acid desaturases and alleles therefor Plant fatty acid desaturases and alleles therefor

2006-07-25

2006-07-25

McElwain, Elizabeth F. (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide alters fat, fatty oil, ester-type wax, or...

C800S298000, C536S023600

Reexamination Certificate

active

07081564

ABSTRACT:
In one aspect, the invention provides new variants of the Fad3 enzyme, including amino acid substitutions, as well as nucleic acid sequences encoding such peptides. Other aspects of the invention include transgenic plants and plant parts. Vectors capable of transforming plant cells are provided, including the nucleic acids of the invention, including Fad3 coding sequences. Corresponding methods are provided for obtaining the transgenic plants of the invention. Methods are provided for using the plants of the invention, including selected plants and transgenic plants, to obtain plant products. Amplification primers for identifying the Fad3 alleles of the invention are provided, together with methods of obtaining plants using the Fad3 alleles of the invention as markers.

REFERENCES:
patent: 4554101 (1985-11-01), Hopp
patent: 4684611 (1987-08-01), Schilperoort et al.
patent: 4743548 (1988-05-01), Crossway et al.
patent: 4801540 (1989-01-01), Hiatt et al.
patent: 4940838 (1990-07-01), Schilperoort et al.
patent: 4943674 (1990-07-01), Houck et al.
patent: 4945050 (1990-07-01), Sanford et al.
patent: 5015580 (1991-05-01), Christou et al.
patent: 5149655 (1992-09-01), McCabe et al.
patent: 5231019 (1993-07-01), Paszkowski et al.
patent: 5464763 (1995-11-01), Schilperoort et al.
patent: 5466587 (1995-11-01), Fitzpatrick-McElligott et al.
patent: 5625130 (1997-04-01), Grant et al.
patent: 5668299 (1997-09-01), DeBonte et al.
patent: 5723765 (1998-03-01), Oliver et al.
patent: 5767338 (1998-06-01), Fan
patent: 5777201 (1998-07-01), Poutre et al.
patent: 5840946 (1998-11-01), Wong et al.
patent: 5850026 (1998-12-01), DeBonte et al.
patent: 0945 514 (1999-09-01), None
patent: 0 255 378 (2002-06-01), None
patent: WO 93/11245 (1993-06-01), None
patent: WO 94/18337 (1994-08-01), None
patent: WO 97/21340 (1997-06-01), None
patent: WO 98/56239 (1998-12-01), None
Somers, D.,J., et al., “Identification of Molecular Markers Associated with Linoleic Acid Desaturation in Brassica Napus”,Theoretical and Applied Genetics, DE, Springer, Berlin, vol. 96, May 1998, pp. 897-903, XP000914248, ISSN: 0040-5752.
Altschul, et al., 1990,J. Mol. Biol. 215:403-10.
Arondel, V., et al., “Map-based cloning of a gene controlling omega-3 fatty acid desaturation in Arabidopsis”,Science258 (5086), 1353-1355 (1992).
Ausubel, et al. (eds.), 1989,Current Protocols in Molecular Biology, vol. 1, Green Publishing Associates, Inc., and John Wiley & Sons, Inc., New York, p. 2.10.3.
Babic, et al., 1998,Plant Cell Rep. 17:183.
Barret, Pierre, et al., “Low Linolenic Acid Level in Rapeseed Can Be Easily Assessed Through the Detection of Two Single Base Substitution in FAD3 Genes”, Abstract.
Bechtold, et al., “In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants”,C.R. Acad. Sci. Paris, Sciences de la vie/Life Sciences1993; 316:1194-9.
Beetham, et al., 1999,Proc. Natl. Acad. Sci. USA96:8774.
Berberich, T., et al., “Two maize genes encoding omega-3 fatty acid desaturases and their differential expression to temperature”,Plant Mol. Biol. 36 (2), 297-306 (1998).
Bhella, R.S., et al., “Nucleotide sequence of a cDNA from Limnanthes douglasii L. encoding a delta-15 linoleic acid desaturase”,Plant Physiol. 108 (2), 861 (1995).
Binding, “Regeneration of Plants, Plant Protoplasts”, CRC Press, Boca Raton, 1985.
Edwards, et al., “A simple and rapid method for the preparation of plant genomic DNA for PCR analysis”,Nucleic Acids Research, vol. 19, No. 6, pp. 1349, 1991.
Evans, et al., “Protoplasts Isolation and Culture”,Handbook of Plant Cell Culture, Macmillian Publishing Company, New York, 1983.
Fraley, et al.,Proc. Nat'l Acad. Sci. USA80:4803 (1983).
Fromm et, al.,Proc. Natl. Acad. Sci. USA82:5824 (1985).
Gamborg and Phillips, “Plant Cell, Tissue and Organ Culture, Fundamental Methods”, Springer Berlin 1995.
Gibson, S., “Cloning of a temperature-regulated gene encoding a chloroplast omega-3 desaturase from Arabidopsis thaliana”,Plant Physiol. 106 (4), 1615-1621 (1994).
Gordon-Kamm, et al. “The Plant Cell” 2:603 (1990).
Hamada, T., et al. “Cloning of a cDNA encoding tobacco omega-3 fatty acid desaturase”,Gene147 (2), 293-394 (1994).
Hamada, T., et al., “cDNA cloning of a wounding-inducible gene encoding a plastid omega-3 fatty acid desaturase from tobacco”,Plant Cell Physiol. 37 (5), 606-611 (1996).
Harlow and Lane, 1988,Antibodies, A Laboratory Manual, Cold Spring Harbor Publications, New York.
Henikoff and Henikoff, 1992,Proc. Natl. Acad. Sci. USA89: 10915-10919.
Horiguchi, G., et al., Developmental regulation of genes for microsome and plastid omega-3 fatty acid desaturases in wheat (Triticum aestivum L.),Plant Cell Physiol. 39, 540-544 (1998).
Horiguchi, G., et al., “Expression of a gene for plastid omega-3 fatty acid desaturases and changes in lipid and fatty acid compositions in light- and dark-grown wheat leaves”,Physiol. Plantarum96, 275-283 (1996).
Horsch, et al.,Science233:496 (1984).
Iba, K., “A gene encoding a chloroplast omega-3 fatty acid desaturase complements alterations in fatty acid desaturation and chloroplast copy number of the fad7 mutant of Arabidopsis thaliana”,J. Biol, Chem. 268 (32), 24099-24105 (1993).
Jourdren, et al., 1996,Theoretical and Applied Genetics93:512.
Kirsch, C., et al., “Rapid, transient, and highly localized induction of plastidial omega-3 fatty acid desaturase mRNA at fungal infection sites in Petroselinum crispum”,Proc. Natl. Acad. Sci. USA. 94 (5), 2079-2084 (1997).
Klee, et al.,Ann. Rev. of Plant Phys. 38:467 (1987).
Klein, et al.,Nature327:70 (1987).
Kodama, H., et al., “Structure, chromosomal location and expression of a rice-gene encoding the microsome omega-3 fatty acid desaturase”,Plant Mol. Biol. 33 (3), 493-502 (1997).
Koncz and Schell, “The promoter of TL-DNA gene 5 controls the tissue- specific expression of chimaeric genes carried by a novel type ofAgrobacteriumbinary vector”,Mol. Gen. Genet. (1986) 204:383-396.
Michaels, et al., 1998, “A robust method for detecting single-nucleotide changes as polymorphic markers by PCR”,The Plant Journal14(3): 381-385.
Moloney, et al., 1989,Plant Cell Rep. 8:238.
Needleman and Wunsch, 1970,J. Mol. Biol. 48:443.
Neff, et al., 1998, “dCAPS, a simple technique for the genetic analysis of single nucleotide polymorphisms: experimental applications in Arabidopsis thaliana genetics”,The Plant Journal14(3): 387-392.
Nishiuchi, T., et al., “Genomic nucleotide sequence of a gene encoding a microsomal omega-3 fatty acid desaturase from Arabidopsis thaliana”,Plant Physiol. 105 (2), 767-768 (1994).
Paszkowski, et al.,EMBO J. 3:2717 (1984).
Pearson and Lipman, 1988,Proc. Natl. Acad. Sci. USA85:2444.
Röbbelen and Nitsch, 1975,L.Z. PfanzenzÜchtg75:93.
Rogers, et al.,Methods Enzymol. 118:627 (1986).
Sakamoto, T., et al., “Temperature-regulated mRNA accumulation and stabilization for fatty acid desaturase genes in the cyanobacterium Synechococcus sp. strain PCC 7002”,Mol. Microbiol. 23 (6), 1281-1292 (1997).
Sakamoto, T., et al., “Cloning of omega 3 desaturase from cyanobacteria and its use in altering the degree of membrane-lipid unsaturation”,Plant Mol. Biol. 26 (1), 249-263 (1994).
Scarth, et al., 1988,Can J. Plant Sci. 68:509.
Scarth, et al., 1994,Can J. Plant Sci75:203.
Smith and Waterman, 1981,Adv. Appl. Math2:482.
Southern, “Detection of Specific Sequences Among DNA Fragments Separated by Gel Electrophoresis”, J. Mol. Biol. (1975) 98, 503-517.
Tang, F., et al., Nucleotide sequence of a cDNA clone for omega-3 fatty acid desaturase (Accession No. AF061027) from Aleurites fordii seeds (PGR99-009),Plant Physiol. 119, 364 (1999).
Tijssen, 1993,Laboratory Techniques in Biochemistry and Molecular Biology—Hybridization with Nucleic Acid Probes, Part I, Chapter

Affiliated with

Also associated with

Rating

Plant fatty acid desaturases and alleles therefor does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Plant fatty acid desaturases and alleles therefor, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Plant fatty acid desaturases and alleles therefor will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3584918