Plant-derived enzyme and DNA sequences and uses thereof

Details Plant-derived enzyme and DNA sequences and uses thereof Plant-derived enzyme and DNA sequences and uses thereof

1998-03-26

2000-05-23

Arthur, Lisa B.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C12Q 168

Patent

active

060664568

ABSTRACT:
The chemically-inducible 27 kD subunit of the enzyme glutathione-S-transferase, isoform II (GST-II-27) and sequences encoding it are provided. In particular, a genomic DNA sequence encoding the gene promoter for the GST-II-27 subunit is provided. Then linked to an exogenous gene and introduced into a plant by transformation, and GST-II-27 promoter provides a means for the external regulation of expression of that exogenous gene. Transformation with DNA encoding glutathione-S-transferase polypeptides produces herbicide resistance transgenic plants.

REFERENCES:
patent: 5073677 (1991-12-01), Helmer et al.
Biochemistry, vol. 22, No. 5, 1983, pp. 1068-1072, Mozer, T.J., et al., "Purification and characterization of corn glutathione S-transferase".
Plant Molecular Biology, vol. 6, No. 4, 1986, pp. 203-211, Shah et al., Structural analysis of glutathione-S-transferase involved in herbicide detoxification.
Biotechnology, vol. 3, Jul. 1985, pp. 629-635, Fraley, et al., "The SEV system: a new disarmed Ti plasmid vector system for plant transformation".
Gene, vol. 76, 1989, pp. 153-160, Wosnick et al., "Total chemical synthesis and expression in Escherichia coli of a maize glutathione-transferase (GST) gene".
Nucleic Acids Research, vol. 14, No. 18, 1986, pp. 7227-7235 Moore et al., "Cloning and expression of a cDNA encoding a maize glutathione-S-transferase gene in E. coli".
Physiol. Plant, vol 77, No. 3, 1989, pp. 465-471 Timmerman, K.P., "Molecular characterization of corn glutathione S-transferase isozymes involved in herbicide detoxification".
Nucleic Acids Research, vol. 16, No. 2, 1988, pp. 425-438, Grove et al., "Characterization and heterospecific expression of cDNA clones of genes in the maize GSH S-transferase multigene family".
J. Cell. Biochem. Suppl., Meeting Apr. 10-16, 1992, vol. 16F, 1992, p. 232, Abstract Y412, Greenland et al., "Cloning and analysis of a cDNA clone encoding a glutathione-S-transferase subunit from maize which is induced by treatment with herbicide safeners".

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