Plant cells and plants expressing chimeric isoprenoid synthases

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S419000

Reexamination Certificate

active

09514513

ABSTRACT:
The invention features plant cells and plants that include a nucleic acid molecule encoding a chimeric isoprenoid synthase polypeptide including an asymmetrically positioned homologous domain. The chimeric isoprenoid synthases of the invention catalyze the production of isoprenoid reaction products that are not produced when the asymmetrically positioned homologous domain is positioned at its naturally-occurring site in an isoprenoid synthase polypeptide.

REFERENCES:
patent: 5744341 (1998-04-01), Cunningham, Jr. et al.
patent: 5824774 (1998-10-01), Chappell et al.
patent: 6072045 (2000-06-01), Chappell et al.
patent: WO 94/22304 (1994-10-01), None
patent: WO 95/11913 (1995-05-01), None
Dudareva N. et al., The Plant Cell, May 2003, vol. 15, pp. 1227-1241.
Dudareva N. et al., The Plant Cell, May 2003, vol. 15, pp. 1227-1241.
Schalk et al., PNAS, 97; (22); pp. 11948-11953.
El Tamer, M et al., Arch. Biochem. Biophvs., 2003; vol. 411, pp. 196-203.
Alonso et al., “Purification of 4S-Limonene Synthase, a Monoterpene Cyclase from the Glandular Trichomes of peppermint (Mentha x piperita) and Spearmint (Mentha spicata),”J. Biol. Chem. 267:7582-7587 (1992).
Anke and Sterner, “Comparison of the Antimicrobial and Cytotoxic Activities of Twenty Unsaturated Sesquiterpene Dialdehydes from Plants and Mushrooms,”Planta Med. 57:344-346 (1991).
Ave et al., “Aphid repellent sesquiterpenes in glandular trichomes ofSolanum berthaultiiandS. tuberosum,”Entomologia Experimentalis et Applicata44:131-138 (1987).
Back et al., “Expression of a Plant Sesquiterpene Cyclase Gene inEscherichia coli,”Arch. Biochem. Biophys. 315:527-532 (1994).
Back et al. “Cloning and Bacterial Expression of a Sesquiterpene Cyclase fromHyoscyamus muticusand Its Molecular Comparison to Related Terpene Cyclases,”J. Biol. Chem. 270:7375-7381 (1995).
Barnby et al. “Effects of Azadirachtin on Levels of Ecdysteroids and Prothoracicotropic Hormone-Like Activity inHeliothis Virescens(Fabr.) Larvae,”J. Insect. Physiol. 36:125-131 (1990).
Borman, “Scientists Mobilize to Increase Supply of Anticancer Drug Taxol,”Chemical&Engineering News11-18 (1991).
Bowers et al., “Sesquiterpene Progenitor, Germacrene A: An Alarm Pheromone in Aphids,”Science196:680-681 (1977).
Cane et al. “Partial Purification and Characterization of Pentalenene Synthase,”Arch. Biochem. Biophys. 254:421-429 (1987).
Cane et al., “Pentalenene Synthase. Purification, Molecular Cloning, Sequencing, and High-Level Expression inEscherichia coliof a Terpenoid Cyclase fromStreptomycesUC 5319,”Biochemistry33:5846-5857 (1994).
Chappell et al., “Accumulation of Capsidiol in Tobacco Cell Cultures Treated With Fungal Elicitor,”Phytochemistry26:2259-2260 (1987).
Chappell, “Biochemistry and Molecular Biology of the Isoprenoid Biosynthetic Pathway in Plants,”Annu. Rev. Plant Physiol. Plant Mol. Biol. 46:521-547 (1995).
Chappell et al., “Is the Reaction Catalyzed by 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase a Rate-Limiting Step for Isoprenoid Biosynthesis in Plants,”Plant Physiol. 109:1337-1343 (1995).
Chappell, “The Biochemistry and Molecular Biology of Isoprenoid Metabolism,”Plant Physiol. 107:1-6 (1995).
Chen et al., “Cloning, Expression, and Characterization of (+)-σ-Cadinene Synthase: A Catalyst for Cotton Phytoalexin Biosynthesis,”Arch. Biochem. Biophys. 324:255-266 (1995).
Chiu et al., “Engineered GFP as a vital reporter in plants,”Current Biology6:325-330 (1996).
Colby et al., “4S-Limonene Synthase from the Oil Glands of Spearmint (Mentha spicata),”J. Biol. Chem. 268:23016-23024 (1993).
Cortes et al., “Repositioning of a Domain in a Modular Polyketide Synthase to Promote Specific Chain Cleavage,”Science268:1487-1489 (1995).
Croteau et al., “Biosynthesis of Monoterpenes: Partial Purification, Characterization, and Mechanism of Action of 1,8-Cineole Synthase,”Arch. Biochem. Biophys. 309:184-192 (1994).
Dixon et al., “Phytoalexin Induction in French Bean,”Plant Physiol. 71:251-256 (1983).
El-Feraly et al., “EPI-Deoxyarteannuin B and 6,7-Dehydroartemisinic Acid fromArtemisia Annua,”J. Nat. Prod. 52:196-198 (1989).
Elakovich, “Sesquiterpenes as Phytoalexins and Allelopathic Agents,”Ecology and Metabolism of Plant Lipids325:93-108 (1986).
Enzell et al., “Mass spectra of tobacco isoprenoids,”Mass Spectrometry Rev. 3:395 (1984).
Facchini et al., “Gene Family for an Elicitor-Induced Sesquiterpene Cyclase in Tobacco,”Proc. Natl. Acad. Sci. USA89:11088-11092 (1992).
Frohman et al., “Rapid production of full-length cDNAs from rare transcripts: Amplification using a single gene-specific oligonucleotide primer,”Proc. Natl. Acad. Sci. USA85:8998-9002 (1988).
Gambliel et al., “Pinene Cyclases I and II,”J. Biol. Chem. 259:740-748 (1984).
Gibson et al., “Wild potato repels aphids by release of aphid alarm pheromone,”Nature302:608-609 (1983).
Guo et al., “Biosynthesis of the Diterpene cis-Abienol in Cell-Free Extracts of Tobacco Trichomes,”Arch. Biochem. Biophys. 308:103-108 (1994).
Habtermariam et al., “A New Antibacterial Sesquiterpene FromPremna Oligotricha,”J. Natl. Prod. 56:140-143 (1993).
Hohn et al., “Isolation and nucleotide sequence of a sesquiterpene cyclase gene from the trichothecene-producing fungusFusarium sporotrichioides,”Gene79:131-138 (1989).
Hohn et al., “Puruification and Characterization of the Sesquiterpene Cyclase Aristolochene Synthase fromPenicillium roqueforti”Arch. Biochem. Biophys. 272:137-143 (1989).
Hohn et al., “Purification and Characterization of the Sesquiterpene Cyclase Trichodiene Synthetase fromFusarium sporotrichioides,”Arch. Biochem. Biophys. 251:756-761 (1986).
Joly et al., “Effect of Site-directed Mutagenesis of Conserved Asparate and Arginine Residues upon Farnesyl Diphosphate Synthase Activity,”J. of Biol. Chem. 268:26983-26989 (1993).
Kalsi et al., “Stereostructures of Two Biologically Active Sesquiterpene Lactones fromInula Racemosa,”Phytochemistry28:2093-2096 (1989).
Koepp et al., “Cyclization of Geranylgeranyl Diphosphate to Taxa-4(5), 11(12)-diene Is the Committed Step of Taxol Biosynthesis in Pacific Yew,”J. Biol. Chem. 270:8686-8690 (1995).
Kubo et al., “Potentiation of antifungal activity of sesquiterpene dialdehydes againstCandida albicansand two other fungi,”Experientia48:1162-1164 (1992).
Kurosaki, “Dissociation of Dimeric 6-Hydroxymellein Synthase, a Polyketide Biosynthetic Enzyme in Carrot Cell Extracts, with Loss of Keto-Reducing Activity,”Archives of Biochem. and Biophys. 321:239-244 (1995).
Lee et al., “Sesquiterpene Antitumor Agents: Inhibitors of Cellular Metabolism,”Science196: 533-536 (1977).
Mabry et al., “Sesquiterpene Lactones and Other Terpenoids,”Herbivores, Their Interaction with Secondary Plant Metabolites, 14:501-537 (1979).
McDaniel et al., “Rational design of aromatic polyketide natural products by recombinant assembly of enzymatic subunits,”Nature375:549-554 (1995).
Midland et al., “The Structure of Syringolides 1 and 2, Novel C-Glycosidic Elicitors fromPseudomonas syringaepv. tomato,”J. Org. Chem. 58:2940-2945 (1993).
Moesta et al., “Casbene Synthetase: Regulation of Phytoalexin Biosynthesis inRicinus communisL. Seedlings,”Arch. Biochem. Biophys. 238:325-333 (1985).
Munck et al., “Purification and Characterization of the Sesquiterpene Cyclase Patchoulol Synthase fromPogostemon cablin,”Arch. Biochem. Biophys. 282:58-64 (1990).
Proctor et al., “Isolation, Characterization, and Bacterial Expression of a Sesquiterpenoid Biosynthetic Gene (Aril) fromPenicillium Roqueforti,”J. Biol. Chem. 268:4543-4548 (1993)

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Plant cells and plants expressing chimeric isoprenoid synthases does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Plant cells and plants expressing chimeric isoprenoid synthases, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Plant cells and plants expressing chimeric isoprenoid synthases will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3788709

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.