Plant brassinolide responsive genes and use thereof

Details Plant brassinolide responsive genes and use thereof Plant brassinolide responsive genes and use thereof

2006-11-07

2006-11-07

Baum, Stuart F. (Department: 1638)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

Reexamination Certificate

active

07132524

ABSTRACT:
The objective of the present invention is to isolate and identify genes responsive to plant hormones, such as, brassinolide, and to provide these genes and their use. The present inventors found two novel genes (referred to as OsBLE1 gene and OsBLE2 gene) whose expressions are markedly increased by brassinolide and auxin, using DNA microarray techniques and Northern blotting. Transformed rice plants were produced usingAgrobacteriumEHA101 which comprise antisense polynucleotide against OsBLE1 and OsBLE2 under the control of the CaMV35S promoter in a binary vector pIG121-Hm. As a result, the transformed rice plants exhibited inhibition in stem and leaf growth as compared with controls which carried the vector alone.

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English translation of Yang, G. and Komatsu, S., “cDNA cloning and characterization of a brassinolide-enhanced gene OsBLE II in rice seedlings,” presented at the 24thAnnual Meeting of the Molecular Biology Society of Japan, Abstract No. 2P-082, p. 494, Yokohama, Japan (Nov. 2001).
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