Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1997-02-13
1998-11-03
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
4353201, 435365, 435419, 43525233, 435410, 536 236, 800205, C12N 120, C12N 1500, C07H 2104, A01H 104
Patent
active
058307478
ABSTRACT:
This invention provides plant arabinogalactan proteins (AGPs) and their genes. AGPs were isolated from Nicotiana alata, Nicotiana plumbaginafolia, and Pyrus communis. Amino acid sequences of isolated AGP peptide fragments are presented. Isolated AGP fragments were used to synthesize oligonucleotide probes to prepare oligonucleotide primers for PCR or prepare RNA probes to screen cDNA libraries of N. alata, N. plumbaginafolia, and P. communis. cDNA clones encoding amino acid sequences of isolated AGP fragments were isolated. The invention presents for the first time an intact AGP amino acid sequence derived from a corresponding AGP gene. The instant invention further provides methods useful in obtaining AGP genes encoding an AGP peptide comprising a specific isolated hydroxyproline-rich (OAST-rich) sequence or a specific isolated hydroxyproline-poor sequence.
REFERENCES:
Du, H. et al. (1994), "Isolation of the Protein Backbone of an Arabinogalactan-Protein from the Styles of Nicotiana alata and Characterization of a Corresponding cDNA," Plant Cell 6:1643-1653.
Che, C.-G. et al. (1994), "Molecular cloning of a gene encoding an arabinogalactan-protein from pear (Pyrus communis) cell suspension culture," Proc. Natl. Acad. Sci. USA 91:10305-10309.
Chen et al. PNAS, 91 : 10305-9, 1994.
Bacic Antony
Chen Chao-Guang
Clarke Adrienne E.
Du He
Gane Alison M.
Cooperative Research Centre For Industrial Plant Biopolymers
Saidha Tekchand
Wax Robert A.
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