Placenta-derived anticoagulating substance

Details Placenta-derived anticoagulating substance Placenta-derived anticoagulating substance

1987-10-02

1989-10-10

Brown, Johnnie R.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

514 2, 424105, A61K 3702, A61K 3550

Patent

active

048732222

ABSTRACT:
A human placenta-derived antiocoagulating substance is prepared by homogenizing a human plancenta, subjecting the resulting homogenate to centrifugal separation, extracting the thus-obtained sediment with a chelating agent, and then separating and purifying the extract. The substance has a molecular weight of 73,000.+-.2,000 as measured in reduced and non-reduced states by SDS-polyacrylamide gel electrophoresis. Its isoelectric point ranges from 6.2 to 6.6 as measured by isoelectric column electrophoresis using an ampholyte. It is inactivated by a heat treatment at 50.degree. C. for 30 minutes, is stable in a pH range of 5.5-8.5 (37.degree. C.) and is also stable in plasma at 37.degree. C. for 15 minutes. It can prolong the recalcification time, prothrombin time and activated partial thromboplastic time.

REFERENCES:
patent: 4585654 (1986-04-01), Landaburu et al.
patent: 4638050 (1987-03-01), Aoki et al.
patent: 4732891 (1988-03-01), Maki et al.
Salem et al., "Isolation and Characterization of Thrombomodulin from Human Placenta" Journal of Bio. Chem. 259(19), 1984, pp. 12246-12251.
Chem Abstract, vol. 92, No. 11, Mar. 17, 1980, p. 355, Abstract No. 91573c, "Anticoagulant Activity of Peptides from the Human Placenta".
The Journal of Biological Chem., vol. 259, No. 19, Oct. 19, 1984, pp. 12246-12251, "Isolation and Characterization of Thrombomodulin . . . ".
Chem Abstract, vol. 100, No. 11, Mar. 12, 1984, p. 258, Abstract No. 82228a, "Purification and Characterization of Human Placental Proteins . . . ".
Chem Abstract, vol. 101, No. 9, Aug. 27, 1984, p. 428, Abstract No. 70045w, "Comparative Studies on Coagulation and Fibrinolytic Activities . . . ".
Chem Abstract, vol. 102, No. 17, Apr. 29, 1985, p. 389, Abstract No. 146724k, "Preparation of Thrombomodulin from Human Placenta".

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