Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2008-09-23
2008-09-23
Goldberg, Jeanine A (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S091200, C536S023100
Reexamination Certificate
active
10930301
ABSTRACT:
A novel T-type calcium channel (CACNA1G) is provided, as are polynucleotides encoding the same. CACNA1G has been implicated in cellular proliferative disorders. More specifically, it has been observed that the methylation state of specific regions within CpG islands associated with the CACNA1G gene correlates with a number of cancerous phenotypes involving a variety of tissue and cell types. Also provided are methods for detecting cellular proliferative disorders by determining the methylation state of genes or regulatory regions associated therewith, including CACNA1G, as well as kits containing reagents for performing invention methods.
REFERENCES:
patent: 5501964 (1996-03-01), Wigler et al.
patent: 5552277 (1996-09-01), Nelson
patent: 5756668 (1998-05-01), Baylin
patent: 5786146 (1998-07-01), Herman et al.
patent: 5912147 (1999-06-01), Stoler et al.
patent: WO 96/35704 (1996-11-01), None
patent: WO 97/45560 (1997-12-01), None
Bayline et al (Advances in Cancer Research, vol. 72, p. 141-196, 1998).
Esteller et al. (Cancer Research, vol. 58, pp. 4515-4518, Oct. 1998).
GeneCards for PITX2; www.genecards.org).
Toyota et al. Cancer Research, vol. 59, pp. 4535-4541, Sep. 1999.
Pao et al. (Human Molecular Genetics, vol. 10, No. 9, pp. 903-910).
Cameron et al. (Blood, vol. 94, No. 7, pp. 2445-2451, Oct. 1999) x.
Toyota, et al., Methylation profiling in acute myeloid leukemia. Blood. May 2001, vol. 97 No. 9, pp. 2823-2828.
Perez-Reyes et al., “Molecular characterization of a neuronal low-voltage-activated T-type calcium channel,”Nature391:896-900 (1998).
Perez-Reyes et al., “Molecular Characterization of Two Members of the T-Type Calcium Channel Family,”Annals New York Academy of Sciences868:131-143 (1999).
Toyota et al., “Inactivation of CACNA1G, a T-type calcium channel gene, by aberrant methylation of its 5′ CpG island in human tumors”.Database Medline US Library of Medicine, AN AF124351 (1999).
West, et al., “Hypomethylation of the Amyloid Precursor Protein Gene in the Brain on Alzheimer's Disease Patient”Journal of Molecular Neuroscience6 (2):141-146 (199.
Zrihan-Licht, et al., “DNA Methylation Status of theMUC1 Gene Cod for a Breast-Cancer-Associated Protein”Int. J. Cancer62:245-251 (1995).
Merlo and Herman, DNA Methylation and Inactivation of Tumor Suppressor Genes'In: Hereditary Cancer, Second International Research Conference on Familial CanceEdited by Hj. Miller, et al., 152-160 (1996).
Ahuja, et al., “Association between CpG island methylation and microsatellite instabi in colorectal cancer”Proceedings of the American Association for Cancer Research38:360-361 (1997).
Shen L, et al, “DNA methylation and environmental exposures in human hepatocellular carcinoma”,J Natl Cancer Inst, vol. 94, No. 10, 755-61, May 15, 1994 (Abstract only).
EMBL; “Homo sapiensT-type calcium channel alpha 1 G (CACNA1G) mRNA, partial cds.”,Database EBI Online!, Jun. 3, 1999 (Database accession No. AF124351 XP-002223698).
“Voltage-dependent T-type calcium channel alpha-1 G subunit (NBR13)”,Database EBI Online!, Jul. 15, 1999, (Database accession No. 043497 XP-002223697).
Mittman, Scott et al., “Structure and alternative splicing of the gene encoding alphall, a human brain T calcium channel alphal subunit”Neuroscience lettersvol. 269, No. 3, 121-124, Jul. 1999.
Mittman, S. et al., “Structure and alternative splicing of the genes encoding the human brain T Ca2= channel subunits alpha1 g and alpha11”Society for Neuroscience Abstracts, vol. 25, No. 1/2, 197, Oct. 1999.
Ueki, Takashi et al., “Hypermethylation of Multiple Genes in Pancreatic Adenocarcinoma”,Cancer Research, vol. 60, 1835-1839, Apr. 1, 2000.
Strathdee, Gordon, et al., “Primary Ovarian Carcinomas Display Multiple Methylator Phenotypes Involving Known Tumor Suppressor Genes”,American Journal of Pathology, vol. 158, No. 3, 1121-1127, Mar. 2001.
Rashid, Asif et al., “CpG Island Methylation in Colorectal Adenomas”,American Journal of Pathology, vol. 159, No. 3, 1129-1135, Sep. 2001.
On-on Chan, Annie et al., “Concordant CpG Island Methylation in Hyperplastic Polyposis”,American Journal of Pathology, vol. 160, No. 2, 529-539, Feb. 2002.
Baylin et al “Alterations in DNA methylation: A fundamental aspect of neoplasia” Advances in Cancer Research, vol. 72, p. 141-196, 1998.
DLA Piper (US) LLP
Goldberg Jeanine A
The Johns Hopkins University School of Medicine
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