Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Patent
1993-06-18
1996-12-17
Green, Lora M.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
436523, 436524, 436527, 436528, 436529, 436531, 530333, 530334, 422 681, 422 99, 422129, G01N 33543, C07K 1700
Patent
active
055852750
ABSTRACT:
Method and apparatus for simple and rapid preparation of reusable, addressable surface-immobilized arrays of biomolecules (libraries) used for screening for interaction with any biologically significant target. A special plate having on or in its surface a plurality of discreet functionalized substrate areas, typically in arrays of 10.times.10 to 400.times.400, is provided for chemical synthesis or bonding thereon of desired families of biomolecules (e.g. peptides, DNA, RNA, oligosaccharides). In the case of peptides, such as hexapeptides, the resulting permanently hexapeptide-loaded plate is a reusable Addressable Synthetic Peptide Combinatorial Library (ASPCL), in which 1 to 3 (typically two) of the positions in the sequence are uniquely identified by the address location. The preferred plate embodiment employs an HPMP wink of porous polyolefin removably received in holes in the plate. A unique multi-slot block assembly is used to prepare the ASPCLs. The wink carrier plate is also employed with a vacuum block system to assist in washing, deprotection, and probing. In library applications, for example determining peptides which bind to functional proteins (enzymes, receptors, antibodies), the substrate-bound peptides are assembled with several positions consisting of uniformly distributed equimolar mixtures of residues, and 2 separated or sequential positions uniquely identified by their spatial location on the substrate array, the "address". Following identification of the known residues giving the greatest affinity for the arrayed positions in the sequence, optimal binding for the complete peptide sequence is determined by an iterative process replacing formerly mixed positions with known AAs at unique addresses.
REFERENCES:
patent: 4631211 (1986-12-01), Houghten
patent: 4777021 (1988-10-01), Wertz et al.
patent: 4965087 (1990-10-01), Wolfbeis et al.
patent: 5010175 (1991-04-01), Rutter et al.
patent: 5100626 (1992-03-01), Levin
patent: 5188733 (1993-02-01), Wang et al.
Frank et al., "Facile and rapid `spot-synthesis` of large numbers of peptides on membrane sheets", pp. 151-152 in Peptides 1990, ed. by Giralt et al., (1991) ESCOM Science Publishers B.V.
Schnorrenberg et al. Tetrahedron, 45, #24, pp. 7759-7764 (1989) "Fully Automatic Simultaneous Multiple Peptide Synthsis in Micromolar Scale-Rapid Synthesis of Series of Peptides for Screening in Biological Assays".
"Light-Directed, Spatially Addressable Parallel Chemical Synthesis" by Steph P. A. Fodor, J. Leighton Read, Michael C. Pirrung, Lubert Stryer, Amy Tsai and Dennis Solas, Research Article, Feb. 15, 1991, p. 767.
Generation and Use of Synthetic peptide Combinatorial Libraries for Basic Research and Drug Discovery by Richard A. Houghten, Clemencia Pinilla, Syl E. Blondelle, Jon R. Appel, Colette T. Dooley and Julio H. Cuervo, Nature, vol. 354, Nov. 7, 1992, p. 84.
Science and Technology--The Silver Shotguns, The Economist, Dec. 14, p. 119 .
Giebel Lutz
Hudson Derek
Johnson Charles R.
Arris Pharmaceutical Corporation
Green Lora M.
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