Piezoelectric device for detection of polynucleotide hybridizati

Details Piezoelectric device for detection of polynucleotide hybridizati Piezoelectric device for detection of polynucleotide hybridizati

1989-08-11

1997-01-21

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

435 6, 4352852, 310311, 310312, 935 86, C12M 134, G21D 702, H02K 4100, C12N 1500

Patent

active

055959083

ABSTRACT:
A system for detecting polynucleotide hybridization is disclosed. A polynucleotide is immobilized on a surface of a piezoelectric crystal. The resonance frequency of the piezoelectric crystal is measured through means for determining the resonant frequency of a piezoelectric crystal. A separate source of polynucleotide is exposed to the polynucleotide-coated piezoelectric crystal for a sufficient length of time and under conditions suitable for hybridization. The resonance frequency of the crystal is then again measured, and the difference between the resonance frequency before and after the incubation period will indicate the extent of hybridization.

REFERENCES:
patent: 4236893 (1980-12-01), Rice
patent: 4242096 (1980-12-01), Oliveira
patent: 4314821 (1982-02-01), Rice
patent: 4376110 (1980-08-01), David et al.
patent: 4486539 (1982-10-01), Ranki et al.
patent: 4591550 (1984-04-01), Hafeman et al.
patent: 4788466 (1988-11-01), Paul et al.
Brenner, D., Prog. Clin. Pathol. 7 (1978) 71.
Huang, E. and J. Pagano, Methods in Virology, K. Maramorosch and H. Koprauski (Eds.), Academic Press, New York, pp. 457-497 (1977).
Stalhandske, et al., J. Clin. Microbiol. 15, (1982) 744.
Ranki, et al., Gene, 21 (1983) 77.
Boileau, et al., J. Clin. Microbiol., 20:959-961 (1984).
Vertanen, et al., J. Clin. Microbiol., 20:1083-1088 (1984).
Bauman, et al., J. Histochem. Cytochem., 29 (1981) 227.
Bauman, et al., J. Histochem. Cytochem., 29 (1981) 238.
Manuelidis, et al., J. Cell. Biol., 95 (1982) 619.
Rudkin et al., Nature, 265 (1977) 472.
Van Prooijen-Knegt, et al., Exp. Cell Res., 141 (1982) 397.
Nakane, et al., J. Cell. Biol., 67 (1975) 302a.
Roederer, et al., Anal. Chem., 55:2333-2336 (1983).
Gilbault, G. G., Anal. Chem., 55:1682-1684 (1983).
Hlavay, et al., Anal. Chem., 49:1890-1898 (1977).
Bioprocessing Technology, 7(3):4, (Mar. 1985).
Wang, Chem. Abst., 103:277 (1985): Abstract No. 155873f.
Olsiewski, P., et al., Am. Biotech. Lab. 3:47-49 (1985).
Tenover, F., Clin. Microbiol. Rev. 1:82-101 (1988).
Wetherall, B. et al., J. Med. Microbiol. 26:257-263 (1988).
Fitts, R., et al., Appl. Environ. Microbiol. 46:1146-1151 (1983).
Drozda, K., Thesis--Master of Science, university of Southern Mississippi (1985).
Hubbard, D. C. et al., Am. Chem. Soc. (Joint Southeast/Southwest Regional) Memphis, TN; Abstr. No. 99, Oct. 10 (1985).
Chien, L. C., et al., Am. Chem. Soc. (Joint Southeast/Southwest Regional) Memphis, TN; Abstr. No. 100; Oct. 10 (1985).
Chien, L. C., et al., J. Miss. Acad. Sci, 31:24 (1986).
Towery, R. B. et al., J. Miss. Acad. Sci., 31:25 (1986).
Flowers, N., et al., J. Miss. Acad. Sci., 31:27 (1986a).
Hubbard, D. C., et al., J. Miss. Acad. Sci., 31:33 (1986).
Fawcett, N. C., et al., Proceed. 4th Army Research Office Biodetection Workshop, pp. 14-15; Cashiers, NC; Apr. 16-18 (1986).
Flowers, N., et al., Fed. Proceed., 45:1516 (1986b).
Walker, D. M., et al., J. Miss. Acad. Sci., 32:15 (1987).
Fawcett, N. C., Evans, J. A. Council For Chemical Research--1st Workshop on Technology Transfer; Denver, CO; Apr. 4-5 (1987).
Fawcett, N. C., et al., 193rd National Meeting--American Chemical Society; Denver, CO; Apr. 8 (1987).
Evans, J. A., et al., American Chemical Society (39th Southeast Regional Meeting); Orlando, FL; Nov. 5 (1987).
Towery, R. B., et al., J. Miss. Acad. Sci., 33:22 (1988).
Chien, L. C., et al., J. Miss. Acad. Sci., 33:23 (1988).
Thompson, R. L., et al., J. Miss. Acad. Sci., 33:24 (1988a).
Fawcett, N. C. et al., Am. Chem. Soc. (Joint 3rd Chem. Cong. of N. Am.-195th Nat. Meet.); Toronto, Canada; Jun. 9 (1988a).
Fawcett, N. C. et al., Anal. Lett., 21:1099-1114 (1988b).
Thompson, R. L., et al., Am. Chem. Soc. (40th Southeast Reg. Meet.) Atlanta, GA; Nov. 10 (1988b).
Chien, L. C., dissertation (PL.D.) "Polymer Reactions for Grafting of Gene Probes Onto Piezoelectric Crystals as Biosensors," U. So. Miss. (1989).
Sensor Tech. 4:No. 10; Oct. (1988).
Cantor, C. R. and Schimmol, P. R., Biophysical Chemistry, Part 1: The Conformation of Biological Macromolecules, Freeman and Company, San Franciso, 1:311 (1980).
van Oss, C. J. and Absolom, D. R., Molecular Immunology, Marcel Decker, Inc., New York, 337-360 (1984).
Hawley's Condensed Chemical Dictionary, Sax et al., eds., Van Nostrand Reinhold, New York, published 1987, pp. 842-843.
Wang. Yichuan 1985, 7(2), 46-48.

Affiliated with

Also associated with

Rating

Piezoelectric device for detection of polynucleotide hybridizati does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Piezoelectric device for detection of polynucleotide hybridizati, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Piezoelectric device for detection of polynucleotide hybridizati will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2323577