Pichia transformation

Details Pichia transformation Pichia transformation

1987-10-19

1990-05-29

Teskin, Robin Lyn

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435255, 435256, 435320, 4351721, 935 55, 935 56, 935 69, C12N 1500, C12N 500

Patent

active

049295551

ABSTRACT:
The present invention provides a method for making whole cells of methylotrophic species of genus Pichia competent for transformation by DNA and a method for transforming with DNA whole cells of such species.

REFERENCES:
Cregg et al, Mol. Cell Biol., vol. 5, pp. 3376-3385, 1985.
J. Bact. Jan. 1983, pp. 163-168--"Transformation of Intact Yeast Cells Treated with Alkali Cations" by Hisao Ito et al.
Current Genetics (1985) 10:39-48--"Integrative Transformation of the Yeast Yarrowia lipolytica" by Lance S. Davidow et al.
Mol Gen. Genet (1986) 202:302-308--"Transformation of the Methylotrophic Yeast Hansenula polymorpha by Autonomous Replication and Integration Vectors" by Rainer Roggenkamp et al.
Molecular and Cellular Biol. Jan 1986, pp. 80-89--"Replicating Plasmids in Schizosaccharomyces pombe: Improvement of Symmetric Segregation by a New Genetic Element" by Wolf-Dietrich Heyer et al.
J. Basic Microbiol. 25 (1985) 2, 141-144--"Transformation of the Industrially Important Yeasts Candida maltosa and Pichia guilliermondii" by G. Kunze et al.
J. Bacteriology, Jun. 1984, pp. 1165-1167--"Transformation of Kluyveromyces fragilis" by Sunil Das et al.

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