Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2009-06-02
2010-06-22
Nashed, Nashaat T (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S252300, C435S254100, C435S325000, C435S320100, C536S023100
Reexamination Certificate
active
07741075
ABSTRACT:
The present invention is directed to recombinant nucleic acid constructs comprising nucleic acid sequences encoding aPichia pastorisPIR secretion signal peptide for secretion or an anchor domain peptide for surface display of a heterologous polypeptide. The present invention is further directed to methods for obtaining a heterologous polypeptide secreted into the culture medium or displayed on the cell surface. The present invention is also directed to transformed cells containing the recombinant nucleic acid constructs.
REFERENCES:
patent: 6107057 (2000-08-01), Crawford et al.
patent: 7125973 (2006-10-01), Lok
patent: 2004/0146976 (2004-07-01), Wittrup et al.
Tanino et al., “Development of Yeast Cells DisplayingCandida AntarcticaLipase B and Their Application to Ester Synthesis Reaction”, Appl. Microbiol Biotechnol, 2007, pp. 1319-1325, vol. 75, Springer.
Zhu et al., “The Surface Display of Haemolysin fromVibrio harveyion Yeast Cells and Their Potential Applications as Live Vaccine in Marine Fish”, ScienceDirect Vaccine, 2006, pp. 6046-6052, vol. 24, Elsevier.
Boder et al., “Yeast Surface Display for Screening Combinatorial Polypeptide Libraries”, Nature Biotechnology, Jun. 1997, pp. 553-557, vol. 15, 1997 Nature Publishing Group.
Chao et al., “Isolating and Engineering Human Antibodies Using Yeast Surface Display”, Nature Protocols, 2006, pp. 755-768 and Erratum, vol. 1, No. 2.
Cochran et al., “Domain-Level Antibody Epitope Mapping Through Yeast Surface Display of Epidermal Growth Factor Receptor Fragments”, Journal of Immunological Methods, 2004 pp. 147-158, vol. 287.
Feldhaus et al., “Flow-Cytometric Isolation of Human Antibodies from a NonimmuneSaccharomyces cerevisiaeSurface Display Library”, http://www.nature.com
aturebiotechnology, Feb. 2003, pp. 163-170, vol. 21 2003 Nature Publishing Group.
Kondo et al., “Yeast Cell-Surface Display-Applications of Molecular Display”, Appl. Microbiol Biotechnol, 2004, pp. 28-40, vol. 64.
Murai et al., “Construction of a Starch-Utilizing Yeast by Cell Surface Engineering”, Applied and Environmental Microbiology, Apr. 1997, pp. 1362-1366, vol. 63 No. 4.
Shimma et al., “Constructions of a Library of Human Glycosyltransferases Immobilized in the Cell Wall ofSaccharomyces cerevisiae”, Applied and Environmental Microbiology, Nov. 2006, pp. 7003-7012, vol. 72, No. 11.
Shusta et al., “Directed Evolution of a Stable Scaffold for T-Cell Receptor Engineering”, Research Articles—Nature Biotechnology, Jul. 2000, pp. 754-759, vol. 18.
Cregg et al., “Recent Advances in the Expression of Foreign Genes inPichia pastoris”, Bio/Technology, Aug. 1993, pp. 905-910, vol. 11, 1993 Nature Publishing Group.
Tanino et al., “Construction of aPichia pastorisCell-Surface Display System using Flo1p Anchor System”, Biotechnol Prog. 2006, pp. 989-993, Flolp, vol. 22.
Furukawa et al., “Development of Novel Yeast Cell Surface Display System for Homo-oligomeric Protein by Coexpression of Native and Anchored Subunits”, Biotechnol Prog., 2006, pp. 994-997 vol. 22.
Tshopp et al., “High-Level Secretion of Glycosylated Invertase in the Methylotrophic Yeast,Pichia pastoris” Bio/Technology, Dec. 1987, pp. 1305-1308, vol. 5 1987 Nature Publishing Group.
Ilgen et al., “Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems”, Ger Gellissen, Ed., Year unknown, pp. 152-153, Wiley-Vch Verlag GmbH and Co. KGaA.
Vedvick et al., “High-Level Secretion of Biologically Active Aprotinin from the YeastPichia pastoris”, J.Ind Microbiol., 1991, pp. 197-201, vol. 7 Elsevier.
Clare et al., “Production of Mouse Epidermal Growth Factor in Yeast: High-Level Secretion UsingPichia pastorisStrains Containing Multiple Gene Copies”, Gene, 1991, pp. 205-212, vol. 105 Elsevier.
Wang et al., “Construction of a NovelPichia pastorisCell-Surface Display System Based on the Cell Wall Protein Pir1”, Curr. Microbiol., 2008, pp. 352-357, vol. 56.
Mergler et al., “Development of a Bisphenol A-Adsorbing Yeast by Surface Display of the Kluyveromyces Yellow Enzyme onPichia pastoris”, Appl. Microbiol. Biotechnol, 2004, pp. 418-421, GP1, vol. 63 Springer.
Ecker et al., “Pir Proteins ofSaccharomyces cerevisiaeAre Attached to B-1, 3-Glucan by a New Protein-Carbohydrate Linkage”, Journal of Biological Chemistry, Apr. 28, 2006, pp. 11523-11529, vol. 281 No. 17.
Board of Regents of the University of Nebraska
Nashed Nashaat T
Romano Charles P.
Thompson & Coburn LLP
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