Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Glycoprotein – e.g. – mucins – proteoglycans – etc.
Patent
1988-05-10
1989-07-11
Schain, Howard E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Glycoprotein, e.g., mucins, proteoglycans, etc.
530397, 530413, 530851, 530853, C07K 1514
Patent
active
048473634
ABSTRACT:
A new glycoprotein ZP-0 originating from the oviduct of a mammal having a molecular weight of about 200,000 to 240,000, as determined by SDS-polyacrylamide gel density-gradient electrophoresis having an isoelectric point of about 4 to 6.2, as determined by isoelectric focusing, containing no sub-fragments, as determined by SDS-disk electrophoresis, forming a carbamylation train in two-dimensional electrophoresis (isoelectric focusing, and SDS-polyacrylamide gel density-gradient electrophoresis, and facilitating species-specific bonding of sperm to zonae pellucidae; and a process for production of the above-mentioned glycoprotein comprising, preparing oviduct from a mammal, homogenating the oviduct optionally with a buffer, to obtain a homogenate, obtaining a liquid containing the glycoprotein from the homogenate, adsorbing the glycoprotein onto lectin immobilized on an insoluble support, liberating the glycoprotein from the support, and recovering the glycoprotein.
REFERENCES:
patent: 4430428 (1984-02-01), Fraser et al.
patent: 4524027 (1985-06-01), Bohn
patent: 4558035 (1985-12-01), Johnson
Gamete Research, 1:265-267 (1978), Oikawa.
Gamete Research, 3:217-231 (1980), Gwatkin.
Biochemistry, 2:356-365 (1980), Dunbar.
Biology of Reproduction 30:435-444 (1984), Drell.
Biology of Reproduction, 30: 445-457 (1984), Drell.
Biology of Reproduction, 32: 619-630 (1985), Dunbar.
Modern Cell Biology, 3: 77-111 (1984), Dunbar.
Biology of Reproduction, 24: 1111-1124 (1981), Dunbar.
Bio Science Laboratory
Schain Howard E.
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