Physiologically active complex

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.

Reexamination Certificate

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C424S085100

Reexamination Certificate

active

10668178

ABSTRACT:
Disclosed is a physiologically active complex which comprises a proteinaceous part with TNF activity and a high molecular part bound artificially to the N-terminus of the proteinaceous part. The proteinaceous part in the complex has the amino acid sequence of SEQ ID NO:2 where Xaa is a member selected from the group consisting of asparagine, alanine, arginine, serine, threonine, proline, methionine, and leucine; while the high molecular part in the complex is a homopolymer of polyethylene glycol, copolymer of polyethylene glycol, or a derivative thereof.

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Terlikowski et al., Toxicology 2002, 174: 143-152.
Haranaka et al., “Antitumor Activity of Murine Tumor Necrosis Factor TNF) Against Transplanted Murine Tumors and Heterotranplanted Human Tumors in Nude Mice”,Int. J. Cancer, vol. 34, pp. 263-267 (1984).
Tracey, “Chapter 16: Tumor necrosis Factor-Alpha”, inthe Cytokine Handbook, ed. Thomson, Academic Press, pp. 289-304 (1994).

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