Drug – bio-affecting and body treating compositions – Enzyme or coenzyme containing – Hydrolases
Patent
1977-12-09
1980-09-23
Daus, Donald G.
Drug, bio-affecting and body treating compositions
Enzyme or coenzyme containing
Hydrolases
128 11, 424 1, 424101, 435 2, A61K 3748, A61K 4300, C12B 312, A61K 3514
Patent
active
042243130
ABSTRACT:
A material capable of forming hydrogen bonds or covalent bonds with loading materials incorporated in loaded cells is introduced into the cells with the loading materials to inhibit the destruction of the cell membranes by the loading material after the permeability of the membranes is brought back down to its normal value. In order to provide for the hydrogen bond formation with common loading materials, such as methotrexate, proteins such as albumin and sugars such as sucrose are effective. The time period during which these materials inhibit a destructive effect on the membranes of loaded cells is well defined. Accordingly, when cells are loaded with a medicament for interaction with substances external to the cells in a physiological solution in which they are placed, which materials have no destructive effect on the cell membranes, it is advantageous to load the cells also with a material having such a destructive effect, such as pronase P, and at the same with a sugar or a protein for delaying its effect, so that the medicament will be released by destruction of the cell membranes after a predetermined time. In this manner, by injection into an animal bloodstream of a suspension of loaded cells prepared according to the invention suspended in a physiological solution, the timing of the release of a medicament into the bloodstream can be controlled.
REFERENCES:
patent: 3887698 (1975-06-01), McConnell et al.
U. Zimmerman et al., Biochim. et Biophys. Acta, 436 (1976), 460-474.
U. Zimmerman et al., Biochim. et Biophys. Acta, 375 (1975), 209-219.
U. Zimmermann et al., Chemical Abstracts 85:29784z, _(1976), Enzyme loading f electrically homogeneous human red blood cell ghosts prepared by dielectric breakdown.
U. Zimmermann et al., Chemical Abstracts 82:108324r, (1975), Preparation of erythrocyte ghosts by dielectric breakdown of the cell membrane.
U. Zimmermann et al., Chemical Abstracts 81:75566v, (1974), Reversible dielectric breakdown of cell membranes in electrostatic fields.
Bock Karin
Buers Hermann-Josef
Pilwat Gunter
Zimmermann Ulrich
Daus Donald G.
Eakin M. C.
Kernforschungsanlage Julich Gesellschaft mit beschrankter Haftun
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