Physiological preparation containing loaded cells in suspension

Drug – bio-affecting and body treating compositions – Fermentate of unknown chemical structure – Having a known elemental analysis

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128 11, 128 13, 424 1, 424 94, 424106, 435 2, A61K 3514, A61K 3748, A61K 4300

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042698260

ABSTRACT:
Magnetic substances in particles of a diameter in the range between 1 and 20 nm are suspended in the physiological solution by which cells are loaded with materials when their cell membranes have increased permeability, the magnetic materials being provided in such dosing, that when the cells are loaded, separated from the loading solution and prepared for use in a fresh physiological solution, thereafter injected to a living body, they can be collected and held fast at a predetermined location, for example, at the location of a tumor, by the effect of an external magnetic field. Ferritin, magnetite, cobalt ferrite, nickel ferrite and other ferrimagnetic, ferromagnetic or even paramagnetic compounds can be incorporated into loaded cells for this purpose.

REFERENCES:
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C. Colley et al., Chemical Abstracts 83: 22192e, Liposomes as Carriers in vivo for Methotrexate (1975).
H. Kimelberg, Biochimica et Biophysica Acta, 448, pp. 531-550 (1976), Differential Distribution of Liposome-Entrapped .sup.3 H-Methotrexate and Labelled Lipids after Intravenous Injection in a Primate.
H. Kimelberg et al., Chemical Abstracts 85: 116483u, The Effect of Entrapment in Liposomes on the In Vivo Distribution of .sup.3 H-Methotrexate in a Primate (1976).
U. Zimmermann et al., Chemical Abstracts 82: 108324r, Preparation of Erythrocyte Ghosts by Dielectric Breakdown of the Cell Membrane (1975).
U. Zimmermann et al., Chemical Abstracts 81: 75566v, Reversible Di-Electric Breakdown of Cell Membranes in Electrostatic Fields (1974).
U. Zimmermann et al., Chemical Abstracts 85: 29784z, Enzyme Loading of Electrically Homogeneous Human Red Blood Cell Ghosts prepared by Di-Electric Breakdown (1976).
T. Nakamura et al., J. of Appl. Physics, vol. 42, No. 4, Mar. 1971, Magneto-Medicine: Biological Aspects of Ferromagnetic Fine Particles, pp. 1320-1324.
R. Newbower, IEEE Transactions on Magnetics, vol. MAG-9, No. 3, Sep. 1973, pp. 447-450, Magnetic Fluids in the Blood.

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