Physiological medium for perfusing, preserving and storing...

Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or...

Reexamination Certificate

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C435S001200, C435S001300, C435S002000, C435S406000

Reexamination Certificate

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06946241

ABSTRACT:
A physiological liquid medium is provided having the basic, synergistic components to allow its universal application in preserving cellular and functional integrity in vitro of different cell, tissue and organ types isolated from different mammalian species. The medium comprises an aqueous solution in sterile purified water of: i) a salt component comprising: a) from 100 to 150 mmoles/L of sodium ions, b) from 2.5 to 6.2 mmoles/L of potassium ions, c) from 1.0 to 2.5 mmoles/L of calcium ions, d) from 0.4 to 25 mmoles/L of magnesium ions, and e) from 96 to 126 mmoles/L of chloride ions; ii) a buffer component comprising: f) from 21 to 27 mmoles/L of bicarbonate ions, and g) from 1 to 12 mmoles/L of TES, MOPS or BES; iii) a substrate component comprising: h) 2 to 11 mmoles/L of glucose, I) 50 to 150 μmoles/L of glycerol and j)7 zo 15 μmoles/L of choline; iv) an amino acid component comprising: k) 5 to 400 μmoles/L of glutamate, 1) 5 to 200 μmoles/L of aspartate and m) 100 to 2000 μmoles/L of glutamine; v) a co-enzyme component comprising: n) 1 to 120 nmoles/L of thiamine cocarboxylase; vi) a vitaminoid component comprising: o) 40 to 70 μmoles/l of D- or DL- or L-carnitine; vii) a protein component comprising: p) 5 to 200 m I.U./L of porcine or human insulin.

REFERENCES:
Parr et al., “Interaction between betamethasone and vecuronium”, Brithish Journal of Anaesthesia 67 : 447-451 (1991).
Clements et al., “Preservation of inherent contractility in isolated gut segments from herbivorous and carnivorous marine fish”, Comp. Physiol. B, 168:61-72 (1998).
Gibco BRL Catalogue, p. 72, 1992.
Rees et al., “Effect of RES-DEL RS-C solution on the viability of isolated rat heart preparations over 1-6 hours of cardioplegic arrest”, Proceeding of the Physiological Society of New Zealand 14 :19 (1995).
Rees, Proceedings of the Physiological Society, Feb. 1978, 278, 8-9P.
Rees (1989) Isolated Perfused Organ Preparatoins. Eds. H.J. Doring, H. Dehnert, Biomesstechnik-Verlag March GmbH, vol. 5 pp. 85-94 and pp. 123-132.
Proceedings of the Physiological Society of New Zealand vol. 14 Aug 1995—Abstracts of two articles one by Rees, and one by Rees and Clissold.
Clements and Rees, J Comp Physiol B (1998) 168: 61-72—Preservation of Inherent Contractllity in Isolated Gut Segments from Herbivorous and Carnivorous Marine Fish.
Andrews, Ainswoth and Abernethy—Transactions of the Royal Society of Tropical Medicine and Hygiene (1994) 88, 200-203.
Parr, Robinson, Rees and Galletly—British Journal of Anaesthesia 1991: 67, 447-451 Interaction Between Betamethasone and Vecuronium.

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