Photoprotein with improved bioluminescence

Details Photoprotein with improved bioluminescence Photoprotein with improved bioluminescence

2003-10-21

2009-10-13

Noakes, Suzanne M. (Department: 1656)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

C536S023100, C536S023400, C536S023500, C435S069100, C435S252300, C435S254110, C435S254200, C435S325000, C435S004000, C435S419000

Reexamination Certificate

active

07601805

ABSTRACT:
Disclosed is a photoprotein with improved luminescent activity, the use thereof as calcium indicator and in cell-based assays for the detection and measurement of intracellular calcium.

REFERENCES:
Ngo et al., in The Protein Folding Problem and Tertiary Structure Prediction, 1994, Merz et al. (ed.), Birkhauser, Boston, MA, pp. 433 and 492-495.
Witkowski et al., Biochemistry, 38, 11643-11650, 1999.
Wishart et al., Journal of Biological Chemistry, vol. 270, No. 45, pp. 26782-26785, 1995.
Kisselev, Structure, vol. 10, pp. 8-9, 2002.
Liu et al. (Structure of the Ca2+-regulated photoprotein obelin at 1.7 Å resolution determined directly from its sulfur substructure, Protein Science, vol. 9 Issue 11, pp. 2085-2093).
Svetlana V. Markova, et al.; “Obelin from the Bioluminescent Marine Hydroid Obelia Geniculata: Cloning, Expression, and Comparison of Some Properties with Those of Other CA2 +-Regulated Photoproteins”, Feb. 19, 2002; Biochemistry 2002, vol. 41, No. 7, pp. 2227-2236.
Boris A. Illarionov, et al.; “Recombinant Obelin: Cloning and Expression of cDNA, Purification, and Characterization as Calcium Indicator”; Methods in Enzymology; vol. 305, 2000, pp. 223-249.
Frederick I. Tsuji, et al.; “Molecular Evolution of the Ca2+-Binding Photoproteins of the Hydrozoa”; Photochimistry and Photobiology, vol. 62, No. 4, 1995, pp. 657-661.
S.V. Matveev, et al.; “Genetically Engineered Obelin as a Bioluminescent Label in an Assay for a Peptide”; Analytical Biochemistry, vol. 270, No. 1, May 15, 1999, pp. 69-74.

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